实用医学杂志2017，Vol.33Issue(9)：1402-1404,3.DOI:10.3969/j.issn.1006-5725.2017.09.010
氧化苦参碱对乙肝病毒的抑制作用及对miR-122表达的影响
Inhibitory effect of oxymatrine on hepatitis B virus and its effect on expression of mircroRNA-122
摘要
Abstract
Objective To investigate whether miR-122 is a target of oxymatrine against HBV. Methods HepG2.2.15 cells were incubated with culture medium containing different concentrations of oxymatrine. The cyto-toxicity of oxymatrine was determined by cck-8 assay. The surface antigen of HBV (HBsAg),antigenof HBV (HBeAg)and HBV DNA in supernatant and intracellular miR-122 were determined in HepG2.2.15 cells after incu-bation with culture medium containing oxymatrine for 72 h. Results The survival percentage of HepG2.2.15 cells under different concentrations of oxymatrine was higher than 95% when the concentration of oxymatrine was lower than 4 mg/mL. After treatment with oxymatrine for 72 h,the secretion of HBsAg and HBeAg,the level of HBV DNA in the supernatant were reduced. The intracellular miR-122 in oxymatrine experience group was 3.5 times higher than that in the negative control group. Conclusions Oxymatrine might be used to inhibit viral replication and antigen expression by enhancing the expression of miR-122 in HepG2.2.15 cells.关键词
氧化苦参碱/乙型肝炎病毒/微小核糖核酸122Key words
Oxymatrine/HBV/miR-122引用本文复制引用
张轩,党秀敏,肖倩,李有强,刘健平,王淏..氧化苦参碱对乙肝病毒的抑制作用及对miR-122表达的影响[J].实用医学杂志,2017,33(9):1402-1404,3.基金项目
广东省中医药局基金资助项目(编号:20152140) （编号:20152140）
