实用医学杂志2017，Vol.33Issue(10)：1580-1583,4.DOI:10.3969/j.issn.1006-5725.2017.10.013
钛颗粒诱导松动界膜中滑膜成纤维细胞HIF-1α的表达抑制其对RANKL/OPG信号通路的活化作用
Suppression of RANKL/OPG pathway activation in FLSs in interfacial membrane through up-regulation of HIF-1α induced by Ti particles
摘要
Abstract
Objective To explore the correlation between HIF-1α expression and RANKL/OPG pathway activation of fibroblast-like synoviocytes(FLSs)induced by Ti particles in aseptic loosening interfacial membrane. Method FLSs were extracted from the synovial tissue collected in surgeries and then co-cultured with Ti particles. QRT-PCR and Western blotting were conducted to measure the mRNA and protein expression of RANKL ,OPG and HIF-1αin FLSs at different concentration and time. Results Genes and protein expression levels of RANKL/OPG and HIF-1α were up-regulated with the increase of concentration of Ti particles. Expression of HIF-1α gene and protein increased time-dependently;the mRNA and protein expression of RANKL/OPG increased firstly and then declined alongside the increase of HIF-1αexpression. Conclusions Ti particles induce the up-regulation of HIF-1α expression and activate RANKL/OPG pathway. Up-regulation of HIF-1α may suppress the activation of RANKL/OPG pathway in FLSs of the interfacial membrane induced by Ti particles.关键词
无菌性松动/滑膜成纤维细胞/HIF-1α/钛颗粒Key words
Aseptic loosening/Fibroblast-like synoviocytes/HIF-1α/RANKL/OPG/Ti particles引用本文复制引用
王成强,何盛茂,谢小波,李奇..钛颗粒诱导松动界膜中滑膜成纤维细胞HIF-1α的表达抑制其对RANKL/OPG信号通路的活化作用[J].实用医学杂志,2017,33(10):1580-1583,4.基金项目
广东省科技计划项目资助(编号2014A020212668) （编号2014A020212668）
