实用医学杂志2017，Vol.33Issue(10)：1589-1592,4.DOI:10.3969/j.issn.1006-5725.2017.10.015
1-磷酸鞘氨醇对肺成纤维细胞纤维化发生的影响和机制
The effect of S1P on HLF cell fibrosis and its mechanism
辛慰 1吉庆春 1彭秋月 1王伟阁 1崔新征1
作者信息
摘要
Abstract
Objective To study the effect of S1P on HLF cell fibrosis and its mechanism. Methods (1) The expression of ECM in HLF cells was analyzed by using Western Blot after treatment by S1P(1 μmol/L), FTY720-P(5μmol/L),ponesimod(5μmol/L)and SEW2871(5μmol/L)24 h;(2)The HLF cells were pre-treated using selective S1PR antagonist W146(1 μmol/L),JTE-013(0.2 μmol/L),and TY-52156(1.25 μmol/L)1 h before incubation by S1P and S1PR agonists 24 h and then the expression of ECM was analyzed;(3)The HLF cells were pre-incubated using JTE-013(0.2μmol/L)and TY-52156(1.25μmol/L)for 1 h and then the expression of ECM was analyzedafter being treated by S1P and S1PR agonists 24 h. Results (1)S1P and selective S1P receptor agonist increased the expression of ECM to various extents;(2)The S1P1R antagonist W146 did not affectthe expression of ECM induced by S1P and S1PR agonists and S1P2R antagonist JTE-013 and S1P3R antagonist TY-52156 both decreased the expression of ECM induced by S1P and S1PR agonists;(3)The expression of ECM induced by S1P and S1PR agonists further decreased using both JTE-013 and TY-52156 but not using ponesimod. Conclusion S1P2R and S1P3R are activated under the influence of S1P so as to increase the synthesis of ECM and promote fibrosis gene expression in HLF cells.关键词
肺纤维化/1-磷酸鞘氨醇/HLF细胞/胶原蛋白Ⅰ/弹性蛋白Key words
Pulmonary fibrosis/Sphingosine 1-phosphate/HLF cell/Collagen Ⅰ/Elastin引用本文复制引用
辛慰,吉庆春,彭秋月,王伟阁,崔新征..1-磷酸鞘氨醇对肺成纤维细胞纤维化发生的影响和机制[J].实用医学杂志,2017,33(10):1589-1592,4.
