现代妇产科进展2017，Vol.26Issue(6)：401-404,4.DOI:10.13283/j.cnki.xdfckjz.2017.06.035
BKCa、SK3、TREK-1钾离子通道在晚孕大鼠子宫中的表达及功能研究
Expression and function of contraction-associated potassium channels in late-pregnant rat uterus
摘要
Abstract
Objective:To investigate the expression of contraction-associated potassium channels in late-pregnant rat uterus and its influence on the uterine contraction.Method:Uteri from non-pregnant(NP) and late-pregnant(LP) was isolated and myometrium strips were prepared for measurement of isometric contraction and potassium channels expression and activity using qPCR and Western blot.Result:Western blot and qPCR analysis demonstrated increased protein amount and mRNA expression in late-pregnant uterus compared with non-pregnancy.Oxytocin caused concentration-dependent contraction of myometrium strips that was reduced in late-pregnant rats compared with non-pregnancy.TREK-1 channel inhibitor L-Methionone caused increased contraction after maximum oxytocin pretreatment in late-pregnant rats.Conclusion:The expression of contraction-associated potassium channels BKCa,SK3 and TREK-1 contributes to the quiescent maintenance of late-pregnant.The uterine contraction regulated by TREK-1 channels during pregnancy may be associated with the onset of delivery.关键词
钾离子通道/子宫收缩/妊娠/TREK-1Key words
Potassium channels/Uterine contraction/Pregnancy/TREK-1分类
医药卫生引用本文复制引用
李韵,何文竹,陈磊,苏晶晶,尹宗智,曹云霞..BKCa、SK3、TREK-1钾离子通道在晚孕大鼠子宫中的表达及功能研究[J].现代妇产科进展,2017,26(6):401-404,4.基金项目
国家自然科学基金青年科学基金(No:81300514) （No:81300514）
中国博士后科学基金(No:2016M592039) （No:2016M592039）
安徽省博士后研究人员科研活动基金(No:2015B080) （No:2015B080）
