摘要
Abstract
Objective:To analyze the mRNAs expression diffrences in the eutopic endometria of women with adenomyosis and construct signal net of differentially expressed genes.Method:Eutopic endometria (n=4) were collected from patients with adenomyosis,and control endometria (n=4) were obtained from women without adenomyosis.The expression of mRNAs in both groups were detected by human gene microarray.Pathways analysis were applied to determine the significant,low false positive rate and targeted pathway that the differentially expressed genes involved.Based on the KEGG database,we constructed signal net of genes and got key genes in the net.Result:In total,612 mRNAs were identified with differential expression between endometria from patients with and without adenomyosis.Among these mRNAs,414 were significantly downregulated and 198 were significantly upregulated.Pathway analysis showed that 40 pathways corresponded to up-regulated transcripts and that the enriched pathways included MAPK signaling pathway,focal adhesion,adherens junction,PI3K-Akt signaling pathway.With respect to down-regulated transcripts,there were 39 pathways corresponded and enriched pathways included DNA replication,cell cycle,cytokine-cytokine receptor interaction.We successfully constructed signal net and got key genes,included HDAC2,ACTN4 etc.Conclusion:The expression of mRNAs in the eutopic endometria were obviously different,which may have close relationship with the pathogenesis of adenomyosis.关键词
子宫腺肌病/在位内膜/信使RNA/信号转导网络Key words
Adenomyosis/Eutopic endometria/Messenger RNA/Signal net分类
医药卫生
