中国组织工程研究2017，Vol.21Issue(16)：2502-2508,7.DOI:10.3969/j.issn.2095-4344.2017.16.008
内质网应激对膝骨关节炎大鼠关节软骨细胞的影响
Effect of endoplasmic reticulum stress on chondrocytes in a rat model of knee osteoarthritis
摘要
Abstract
BACKGROUND: Endoplasmic reticulum (ER) stress has been proved to be related to the occurrence of diabetes, dilated cardiomyopathy and neurodegenerative diseases. Indeed, it is closely associated with osteoarthritis. OBJECTIVE: To explore the effect of ER stress on the chondrocyte viability as well as the occurrence and development of osteoarthritis in rats. METHODS: Rat chondrocytes were isolated and cultured, and the ER stress in the rat chondrocytes was by 10 mg/L tunicamycin. The expression levels of ER stress markers C/EBP-homologous protein and 78 kDa glucose-regulated protein were detected by western blot assay, and the proliferation and apoptosis of chondrocytes were detected by cell counting kit-8 assay and AnnexinV-FITC flow cytometry, respectively. In the in vivo experiment, 15 Sprague-Dawley rats were selected and subjected to anterior cruciate ligament transection and medial meniscectomy to establish an animal model of osteoarthritis. Tunicamycin, tauroursodeoxycholic acid and PBS (blank control group) were respectively injected into the articular cavity, and then the progression of osteoarthritis was assessed by hematoxylin-eosin staining at 4 weeks after treatment. RESULTS AND CONCLUSION: After addition of tunicamycin, the expression levels of C/EBP-homologous protein and 78 kDa glucose-regulated protein were significantly upregulated, the viability of chondrocytes was decreased gradually, while the apoptotic rate was increased significantly. Results from gross observation and hematoxylin-eosin staining suggested that tunicamycin promoted the progression of osteoarthritis and tauroursodeoxycholic acid delayed the deterioration of cartilage in the rats. These findings indicate that ER stress results in the decreased chondrocyte viability and increased apoptosis, which may be an important pathogenesis of osteoarthritis. Additionally, tauroursodeoxycholic acid can effectively alleviate osteoarthritis induced by ER stress.关键词
组织构建/软骨细胞/内质网应激/骨关节炎/凋亡/衣霉素/牛磺熊去氧胆酸/体外实验/体内实验/北京市自然科学基金分类
医药卫生引用本文复制引用
吴浩,李翔,李卓扬,孟志超,曹永平,潘利平,周星彤,杨昕,刘恒,王瑞,崔云鹏..内质网应激对膝骨关节炎大鼠关节软骨细胞的影响[J].中国组织工程研究,2017,21(16):2502-2508,7.基金项目
北京市自然科学基金青年基金项目(7144252) Funding:the Natural Science Foundation of Beijing for the Youth, No. 7144252 （7144252）
