中国组织工程研究2017，Vol.21Issue(17)：2638-2643,6.DOI:10.3969/j.issn.2095-4344.2017.17.003
CD54+和CD54-脂肪干细胞的成脂分化能力
Adipogenic capacity of CD54+/CD54- adipose-derived stem cells
摘要
Abstract
BACKGROUND: Studies have shown that adipose-derived stem cells have pluripotent differentiation potential, but only 30%-40% of cells can differentiate into mature adipocytes with low adipogenic differentiation potential. Therefore, how to improve the adipogenic differentiation ability of adipose-derived stem cells is a key problem to be solved in the process of soft tissue regeneration. OBJECTIVE: To observe the relationship between the surface marker CD54 of rabbit adipose-derived stem cells and their adipogenic capacity, and to explore the adipogenic differentiation of CD54+/CD54- adipose-derived stem cells underthe same induction. METHODS: We successfully isolated and cultured the adipose-derived stem cells from inguinal subcutaneous fat pads (3 ml) of New Zealand white rabbits, aged 8-12 weeks, which were induced into multi-differentiation and used to detectsurface markers. We sorted the passage 3 adipose-derived stem cells by immunomagnetic beads and divided into two categories including CD54+ and CD54- adipose-derived stem cells. After 14 days of adipogenic induction, the cells in the two groups were subjected to oil red O staining and were compared by detecting the density of mature adipocytes and lipid droplet contenT.RESULTS AND CONCLUSION: The cultured adipose-derived stem cells possessed the characteristics of mesenchymal stem cells that could differentiate into mature adipocytes, osteoblasts and chondrocytes, with CD29, CD44, CD49d, CD54, CD73, CD90 and CD105 positive expression while CD31, CD34 and CD45 negative expression. Fourteen days after adipogenic induction, the density of mature adipocytes and the intracellular lipid droplet content in the CD54+ group were significantly higher than those in the CD54- group (P < 0.05). We also found that the mRNA expressions of PPARγ,ADD1, C/EBPα related to adipogenic differentiation in the CD54+ group were significantly higher than those in the CD54- group (P < 0.05). Taken together, CD54+ adipose-derived stem cells have excellent adipogenic differentiation capacity.关键词
干细胞/脂肪干细胞/细胞表面标志/成脂分化/细胞诱导/组织工程/国家自然科学基金分类
医药卫生引用本文复制引用
李德全,梁至洁,韦金儒,黄海,黄敏红,池刚毅,黎洪棉..CD54+和CD54-脂肪干细胞的成脂分化能力[J].中国组织工程研究,2017,21(17):2638-2643,6.基金项目
国家自然科学基金资助项目(81560316,81560358) （81560316,81560358）
广西科学研究与技术开发计划资助项目(桂科攻1598012-1) （桂科攻1598012-1）
广西自然科学基金重点项目(2016GXNSFDA380016) （2016GXNSFDA380016）
南宁市科学研究与技术开发计划资助项目(20153089,zc20153002) the National Natural Science Foundation of China, No. 81560316, 81560358 （20153089,zc20153002）
the Scientific Research and Development Plan of Guangxi Zhuang Autonomous Region in China, No. 1598012-1 （）
the Natural Science Foundation of Guangxi Zhuang Autonomous Region, No. 2016GXNSFDA380016 （）
the Science Research and Technology Development Plan of Nanning City, No. 20153089, zc20153002 （）
