中国组织工程研究2017，Vol.21Issue(17)：2659-2664,6.DOI:10.3969/j.issn.2095-4344.2017.17.006
肝衰竭患者血浆置换前后血清诱导脐带间充质干细胞向肝细胞的分化
Hepatic failure patient's serum before and after plasmapheresis induces the differentiation of umbilical cord mesenchymal stem cells into hepatic cells
摘要
Abstract
BACKGROUND: Umbilical cord mesenchymal stem cells (UC-MSCs) have made certain curative effect on hepatic failure, but little is reported on the effect of hepatic failure patient's serum microenvironment on UC-MSCs differentiation ability. OBJECTIVE: To investigate the effect of hepatic failure patient's serum on the differentiation of umbilical cord mesenchymal stem cells into hepatic cells. METHODS: UC-MSCs were isolated by tissue adherent method and the cell morphology and phenotype identified by microscope and flow cytometry. Alpha-MEM media with serum before/after plasmapheresis were used to culture the hirdgeneration of UC-MSCs, and regular fetal bovine serum culture medium acted as control group. Inverted microscope was used to observe the cell morphology in three groups. Immunohistochemical method was used to measure expression level of alpha fetoprotein, albumin and cytokeratin 18. RESULTS AND CONCLUSION: A great amount of high-purity UC-MSCs could be obtained using tissue adherent method, which highly expressed CD44, CD73, CD90, CD10, but did not express CD45. Hepatic failure patient's serum could change the morphology of UC-MSCs and induce UC-MSCs to express alpha fetoprotein, albumin and cytokeratin 18. The positive expression of alpha fetoprotein, albumin and cytokeratin 18 was significantly increased after plasmapheresis (P < 0.05). To conclude, hepatic failure patient's serum after plasmapheresis exert more benefits to induce UC-MSCs to differentiate into hepatic cells than that before plasmapheresis.关键词
干细胞/脐带脐血干细胞/脐带间充质干细胞/血浆置换/肝衰竭/肝细胞分化分类
医药卫生引用本文复制引用
王晶波,耿大影,李晓迎,陈莉,徐凤,史兆章..肝衰竭患者血浆置换前后血清诱导脐带间充质干细胞向肝细胞的分化[J].中国组织工程研究,2017,21(17):2659-2664,6.基金项目
2012 年度济南市科技局无资计划项目 （）
山东省医药卫生科技发展计划项目(2014WS0006) the Non-funded Project of Jinan Science and Technology Department in 2012 （2014WS0006）
the Medical and Health Development Plan of Shandong Province, No. 2014WS0006 （）
