中国组织工程研究2017，Vol.21Issue(17)：2684-2689,6.DOI:10.3969/j.issn.2095-4344.2017.17.010
带部分松质骨小牛皮质骨复合骨髓间充质干细胞植入兔体内成骨及骨形态发生蛋白2的表达
Osteogenesis and expression of bone morphogenetic protein 2 after bone marrow mesenchymal stem cells combined with calf cortical bone with partial cancellous bone implanted into rabbits
摘要
Abstract
BACKGROUND: Previous studies have confirmed that rabbit bone marrow mesenchymal stem cells (BMSCs) can differentiate into osteoblasts under osteogenic induction in vitro, stably express the specific phenotype of osteoblasts and have osteogenic ability. Calf cortical bone scaffold with partial cancellous bone has good biocompatibility and degradability, which can be used as a carrier material of bone marrow mesenchymal stem cells. OBJECTIVE: To combine rabbit BMSCs with calf bone composite according to the basic principles of bone tissue engineering and to observe the osteogenesis in the New Zealand white rabbits after implantation of BMSCs/calf bone composite into the ilium, thereby providing a direct evidence for preliminary clinical application of tissue-engineered bone products.METHODS: BMSCs/calf cortical bone scaffold with partial cancellous bone (tissue-engineered bone group), simple calf heterogeneous bone (heterogeneous bone group) or autologous iliac bone (autologous iliac bone group) was randomly implanted into the rabbit ilium. The changes of implant surface and tissue reactions around the implant were observed.X-ray examination was performed to observe osteogenic changes at 4, 8, 12, 24 weeks after implantation. Immunohistochemistry staining was used to observe the expression of bone morphogenetic protein 2.RESULTS AND CONCLUSION: After heterogeneous bone implantation, the wound healed well, and there were no systemic or local inflammation and toxicity reactions in all groups. The X-ray results showed that at postoperative 24 weeks, the implant was basically fused with the host bone in the tissue-engineered bone group, but the fusion was unsatisfactory in the heterogeneous bone group. The process of ossifications from cartilages was observed in all groups by hematoxylin-eosin staining, and bone morphogenetic protein 2 was positive for immunohistochemical staining. Findings from in vivo experiments indicate that rabbit BMSCs seeded onto the calf cortical bone scaffold with partial cancellous bone could construct tissue-engineered bone by osteoinductation in vitro in the rabbits.关键词
干细胞/骨髓干细胞/骨髓间充质干细胞/带部分松质骨的小牛皮质骨/骨形态发生蛋白2分类
医药卫生引用本文复制引用
陈能,邵云峰,刘傥,张湘生,熊光仲..带部分松质骨小牛皮质骨复合骨髓间充质干细胞植入兔体内成骨及骨形态发生蛋白2的表达[J].中国组织工程研究,2017,21(17):2684-2689,6.基金项目
湖南省科技计划项目(2013FJ6001) （2013FJ6001）
深圳市科创委基金(20150305190447) the Scientific Research Project of Hunan Province, No. 2013FJ6001 （20150305190447）
the Foundation of the Science and Technology Innovation Committee of Shenzhen, No. 20150305190447 （）
