中国组织工程研究2017，Vol.21Issue(17)：2708-2713,6.DOI:10.3969/j.issn.2095-4344.2017.17.014
神经干细胞的培养鉴定及分化
Cultivation, identification and differentiation of neural stem cells
摘要
Abstract
BACKGROUND: Neural stem cell transplantation is an emerging therapeutic option in the recovery of neural lesions and neurodegenerative diseases. Neural stem cell culture and differentiation lay a foundation for the further study. OBJECTIVE: To improve the techniques for the isolation, cultivation, differentiation and identification of neural stem cells, and to explore the biological characteristics of cells. METHODS: The neural stem cells from C57BL/6 fetal rats were isolated and cultured in vitro using neurophere culture method followed by morphological and ultrastucture examination. The growth curve and cell cycle of passage 3 cells were drawn and analyzed. Nestin expression was tested by immunofluorescence. Neural stem cells induced in 1% and 10% fetal bovine serum were identified using anti-GFAP, anti-βⅢ-tubulin and anti-MBP by immunofluorescence. RESULTS AND CONCLUSION: The neurospheres exhibited strong cell proliferation ability. Under transmission electron microscope, there was a high nuclear/cytoplasmic ratio in the neural stem cells, indicating a low differentiation degree. Immunofluorescence analysis revealed that neural stem cells were positive for Nestin. The induced cells were positive for GFAP, βⅢ-tubulin, and MBP, indicating these cells were induced to differentiate into astrocytes, neurons and oligodendrocytes, and there were more neurons in 1% fetal bovine serum than those in 10% fetal bovine serum. In conclusion, we could successfully isolate neural stem cells in C57BL/6 mice, and low concentration of fetal bovine serum contributes to more neurons differentiated from neural stem cells.关键词
干细胞/分化/神经干细胞/培养/鉴定/血清/诱导分化/国家自然科学基金分类
医药卫生引用本文复制引用
朱琼,皋月娟,高顺记,陈重,刘政,徐亚丽..神经干细胞的培养鉴定及分化[J].中国组织工程研究,2017,21(17):2708-2713,6.基金项目
国家自然科学基金面上项目(81471795) （81471795）
全军医学科技青年培育计划(16QNP102) the National Natural Science Foundation of China, No. 81471795 （16QNP102）
the Military Medical Incubation Plan for the Youth, No. 16QNP102 （）
