中国组织工程研究2017，Vol.21Issue(18)：2828-2833,6.DOI:10.3969/j.issn.2095-4344.2017.18.007
低频脉冲电磁场在三维支架下对人脂肪源干细胞增殖和成骨分化的影响
Effect of low-frequency pulsed electromagnetic fields on proliferation and osteogenic ability of human adipose-derived stem cells in a three-dimensional scaffold
摘要
Abstract
BACKGROUND:Nowadays increasing experimental findings have proved that the low-frequency pulsed electromagnetic fields (LPEMF) can induce osteogenic differentiation of a variety of stem cells in the two-dimensional scaffold. However, little is reported on the LPEMF effect on the proliferation and osteogenic differentiation of stem cells in the three-dimensional scaffold.OBJECTIVE:To investigate the effect of LPEMF on the proliferation and osteogenic differentiation of human adipose-derived stem cells (hASCs) in the 3D Insert-PCL scaffold.METHODS:Passage 3 hASCs were directly cultured in the 3D Insert-PCL scaffolds folowed by LPEMF (50 Hz, 1 mT) exposure, 2 hours per day, for continuous 14 days (experimental group) or no intervention (control group). After 7 days of culture, Live/Dead staining was used to observe cell survival. After 1, 3, 5, 7 days of culture, MTT assay was used to detect cell proliferation. After 7 and 14 days of culture, the osteogenic differentiation of hASCs was assessed through the alkaline phosphatase (ALP) staining and qRT-PCR.RESULTS AND CONCLUSION: Live/dead cell staining proved that the hASCs had a good growth in the 3D Insert-PCL scaffolds as well as a high survival rate. The absorbance values of hASCs in the two groups were increased gradualy with time, and the absorbance value in the experimental group was significantly higher than that in the control group at 1 and 3 days after culture (P < 0.05). The ALP activity in the experimental group was stronger than that in the control group at 7 and 14 days after culture. qRT-PCR findings showed that at 7 days after culture, the mRNA levels of ALP and type Ⅰ collagen were significantly higher in the experimental group than the control group (P < 0.01), while at 14 days after culture, the mRNA levels of osteopontin, Runt-related transcription factor, ALP and type Ⅰ collagen were significantly higher in the experimental group than the control group (P < 0.05). To conclude, the LPEMF exposure can promote the proliferation and osteogenic differentiation of hASCs cultured on the the 3D Insert-PCL scaffold.关键词
生物材料/骨生物材料/聚己内酯3D培养支架/低频脉冲电磁场/人脂肪源干细胞/增殖/成骨分化Key words
Electromagnetic Fields/Stem cells/Cell Proliferation/Tissue Engineering分类
医药卫生引用本文复制引用
陈宇雄,陈贤哲,倪梦杉,郭文杰,田京..低频脉冲电磁场在三维支架下对人脂肪源干细胞增殖和成骨分化的影响[J].中国组织工程研究,2017,21(18):2828-2833,6.基金项目
广东省大学生科技创新培育专项资金(pdjh2016b0104).广东省科技计划项目(2013B021800312) the Scientific Innovative Culture Fund for the Undergraduate in Guangdong Province, No. pdjh2016b0104 （pdjh2016b0104）
the Scientific Research Plan of Guangdong Province, No. 2013B021800312 （）
