中国组织工程研究2017，Vol.21Issue(18)：2839-2845,7.DOI:10.3969/j.issn.2095-4344.2017.18.009
新型多孔钽金属支架材料的生物学评价
Biological evaluation of a novel porous tantalum scaffold
摘要
Abstract
BACKGROUND:Based on the excellent biological performance of tantalum, tantalum coating can be prepared on the conventional substrate by the coating preparation technique, which lowers the cost and importantly provides a feasible direction for the application of tantalum metal devices.OBJECTIVE:To study the biocompatibility of porous silicon carbide scaffold with tantalum coating.METHODS: By atmospheric pressure chemical vapor deposition, the porous tantalum-coated silicon carbide scaffold was prepared. In anin vitro experiment, bone marrow mesenchymal stem cells were co-cultured with the porous tantalum-coated silicon carbide scaffold for 2 weeks. Then cell proliferation was detected using MTT method. Cell adhesion on the scaffold was observed using scanning electron microscope at 5, 10, 15 days after culture. In anin vivo experiment, the porous tantalum-coated silicon carbide material was implanted into the region of femoral head defects in dogs. Experimental animals were kiled at 6, 12 weeks after implantation, and then the specimens of hard tissue sections were stained to observe the implant material and growth of its surrounding tissues under the microscope.RESULTS AND CONCLUSION: (1)In vitro experiment results: with increasing time in the co-culture, bone marrow mesenchymal stem cells exhibited very strong proliferation, mutual connection and close arrangement. Interconnected cells crept into the pores of the porous tantalum-coated silicon carbide scaffold, and completely covered the tantalum surface, then forming overlapped cell clusters. (2)In vivo experimental results: 6 weeks after implantation, there was a clear boundary between the implant and surrounding bone tissues, and only a small amount of bone tissues crept into the porous tantalum-coated silicon carbide material in the presence of a little trabecular bone. Some voids and cracks around the pores of the implant were detectable. Neither damage nor rejection of tissues around materials occurred. At 12 weeks after implantation, the implant was fused well with the surrounding tissues in the presence of a large amount of bone tissues growing into the surface and pores and a large number of trabecular bones. To conclude, the tantalum-coated silicon carbide scaffold prepared by the atmospheric pressure chemical vapor deposition has good biocompatibility.关键词
生物材料/材料相容性/碳化硅/多孔钽/化学气相沉积法/骨髓间充质干细胞/生物相容性Key words
Materials Testing/Tantalum/Stem cells/Tissue Engineering分类
医药卫生引用本文复制引用
谢辉,马志杰,王建川,王本杰,王威,尉晓蔚,赵德伟..新型多孔钽金属支架材料的生物学评价[J].中国组织工程研究,2017,21(18):2839-2845,7.基金项目
国家十二五科技支撑项目(2012BAI17B02),项目名称:新一代骨科新型生物医用材料及产品研发.国家十三五"国家重点研发计划"项目(2016YFC1102000),项目名称:生物活性脊柱及节段骨缺损修复器械的产品研发 the Key Projects in the National Science & Technology Pilar Program during the Twelfth Five-Year Plan Period, No. 2012BAI17B02 （2012BAI17B02）
the National Major Research and Development Plan during the Thirteenth Five-Year Plan Period, No. 2016YFC1102000 （）
