中国组织工程研究2017，Vol.21Issue(18)：2846-2851,6.DOI:10.3969/j.issn.2095-4344.2017.18.010
镁合金材料与人内皮细胞的生物相容性
Biocompatibility of magnesium alloys with human endothelial cells
摘要
Abstract
BACKGROUND:Magnesium alloy is a material that is easily degradable and has good mechanical properties.OBJECTIVE:To study the effects of the two kinds of magnesium aloy materials (AM50 and WE43) on proliferation and morphology of human umbilical vein endothelial cells.METHODS: Human umbilical vein endothelial cells at logarithmic phase were cultured in 100%, 50%, 10% extracts of AM50 and WE43 or directly seeded on the surface of AM50 and WE43 for 24 hours to study the morphological effect of magnesium alloys on the cells. Human umbilical vein endothelial cells cultured routinely served as negative controls. Relative growth rate of the cells was detected at 12, 24, 48 hours after culture. Cell morphology was observed using immunofluorescence staining at 24 hours after culture, and pH and element levels in the cell supernatant were measured.RESULTS AND CONCLUSION: (1) AM50 and WE43 extracts showed no effects on the proliferation of human umbilical vein endothelial cells in comparison with the negative control group. (2) Compared with the negative control group, reduced area of human umbilical vein endothelial cells was found on the surface of AM50 or WE43 alloy by the immunofluorescent staining, and the cytoskeleton of the cytoplasm was blurred. However, after 24 hours of culture, morphological changes of the cells cultured in 10%, 50% and 100% AM50 or WE43 extracts were consistent with those cultured routinely. The cytoskeleton of the cytoplasm was clearly visible. (3) Compared with the negative control group, the pH values of 100% AM50 and WE43 extract groups were significantly upregulated, and the level of magnesium element was also increased remarkably. In summary, AM50 and WE43 alloys possess good cytocompatibility with human umbilical vein endothelial cells, and have no influence on the cell proliferation and growth.关键词
生物材料/材料相容性/镁合金/内皮细胞/细胞增殖/生物相容性/口腔生物材料Key words
Dental Aloys/Cell Proliferation/Endothelial cells/Tissue Engineering分类
医药卫生引用本文复制引用
王心玲,胡桐楠,储冰峰..镁合金材料与人内皮细胞的生物相容性[J].中国组织工程研究,2017,21(18):2846-2851,6.基金项目
军队医学科技青年培育项目(130NP198):不同致龋性变形链球菌差异非编码小RNA的筛选与功能研究 the Military Medical Science and Technology Training Project for the Youth, No. 130NP198 （130NP198）
