中国组织工程研究2017，Vol.21Issue(18)：2852-2857,6.DOI:10.3969/j.issn.2095-4344.2017.18.011
新型蜂巢样聚己内酯-硅酸钙复合晶体材料修复颅骨极限缺损
Bone regenerationvia a novel honeycomb-like polycaprolactone-calcium silicate crystal compound scaffold in extreme-sized cranial defects
摘要
Abstract
BACKGROUND:Polycaprolactone as a polymer material has poor biocompatibility, and needs to be combined with other natural biological materials to increase biocompatibility, thereby promoting tissue regeneration.OBJECTIVE:To develop a novel honeycomb-like polycaprolactone-calcium silicate crystal compound scaffold, and observe its osteogenic effects in Sprague-Dawley rats with skull defects.METHODS: Eighteen Sprague-Dawley rats were used to make skull defect models and randomized into three groups: blank control group with no implantation, control group with implantation of normal polycaprolactone-calcium silicate crystal compound scaffold, and experimental group with implantation of the novel honeycomb-like polycaprolactone- calcium silicate crystal compound scaffold. Six weeks after implantation, bone regeneration effect in the defect region measuredvia X-ray scanning, Micro-CT three-dimensional reconstruction, and histological analysis.RESULTS AND CONCLUSION: (1) X-ray scan: in all the rats, the size of bone defect was reduced, the fracture line became vague, and the marginal bone density was increased. The percentage of new bone area was highest in the experimental group, successively followed by the control group and blank control group. (2) Micro-CT three-dimensional reconstruction: new bones in the blank control group were mainly distributed on the both sides of the defect, but those in the control and experimental groups distributed in the defect region. The bone regeneration capacity was ranked as follows: experimental group > control group > blank control group (P < 0.05). (3) Histological analysis: new bone ingrowth was visible in all the three groups to different extents. Compared with the other two groups, new bone formation and microvessel density were significantly higher in the experimental group (P < 0.05). To conclude, this novel honeycomb-like polycaprolactone-calcium silicate crystal compound scaffold can obviously promote bone formation in the skull defect region.关键词
生物材料/骨生物材料/聚己内酯-硅酸钙复合晶体材料/骨缺损/颅骨再生/骨修复/聚己内酯/广东省自然科学基金Key words
Skull/Bone Regeneration/Tissue Engineering分类
医药卫生引用本文复制引用
宋兵,廖哲霆,陈宇璠,赵亮..新型蜂巢样聚己内酯-硅酸钙复合晶体材料修复颅骨极限缺损[J].中国组织工程研究,2017,21(18):2852-2857,6.基金项目
广东省自然科学基金(2014A030313275,s2013010014253).南方医科大学"国家杰出青年培养计划"项目 the Natural Science Foundation of Guangdong Province, No. 2014A030313275, s2013010014253 （2014A030313275,s2013010014253）
the National Outstanding Youth Culture Plan of Southern Medical University （）
