畜牧兽医学报2017，Vol.48Issue(6)：990-999,10.DOI:10.11843/j.issn.0366-6964.2017.06.003
鸡ev21占位区和未占位区重复序列结构与羽速表型关系研究
Relation between Feather Speed and Gene Structure of Repeated Sequences of ev21 Occupied-site and Unoccupied-site Regions in Chicken
摘要
Abstract
The aim of this study was to explore the molecular basis of Hae Ⅲ digestion detection of genotypes of chicken feather speed in chicken.Three hundred and thirteen individuals in 6 strains of chickens (Taihang,Bashang Long-tail,Dawufen,Hy-line Gray,Hy-line Brown,progenitor of Hy-line Gray) with definite phenotype of feather speed were detected with RFLP.The common region of occupied site(OS) and unoccupied site(US) for 538 bp,also the specific region of OS for 1 440 bp,were digested with Hae Ⅲ.The results showed that:1) The fragment for 1 450 bp was the unoccupied-site repeated sequence for ev21 ascertained by Blast.The digestion detection results of Hae Ⅲ for 1 450 bp were completely consistent with the phenotype of HY-line Gray and progenitors of HY-line Gray and Dawufen,but their consistency with Taihang slow-feather cock,Taihang slow-feather hen,Bashang Long-tail slow-feather cock and HY-line Brown slow-feather chicken were 40.0％,27.6％,28.6％ and 0.0％,respectively.2) The consistency was above 92％ between digestion results of 538 bp and phenotype in Taihang and Bashang Long-tail chicken,and the other strains were 100.0％ except for HY-line Brown fast-feather cock with 0.0％.3) The positive rate of 1 440 bp fragment PCR in slow-feather Taihang cock,Taihang hen and fast-feather Taihang cock was 94.1％,65.5％ and 0.0％,respectively,and it was 100.0％ and 0.0％ in slow-feather and fast-feather progenitors of Hy line gray,respectively.The 1 440 bp fragment could not be digested by Hae Ⅲ and it could be a detection basis for ev21.The structure was analyzed comprehensively for the OS and US of ev21 in 6 strains of chicken and it was concluded that the site recognized by Hae Ⅲ enzyme in US could not be a detection base for slow-feather and ev21,while ev21 insertion in OS and the site for Hae Ⅲ digestion in 1 440 bp were linked with the mutation of A → G and 8 bases repeated.关键词
鸡/羽速/占位区/未占位区/ev21/Hae Ⅲ酶切Key words
chicken/feather speed/occupied-site region/unoccupied-site region/ev21/Hae Ⅲ digestion分类
农业科技引用本文复制引用
张乐超,王晗,张秀玲,刘春杨,王麒,周荣艳,李祥龙,李兰会..鸡ev21占位区和未占位区重复序列结构与羽速表型关系研究[J].畜牧兽医学报,2017,48(6):990-999,10.基金项目
河北省现代农业技术体系蛋鸡产业创新团队(HBCT2013090206) （HBCT2013090206）
河北省自然科学基金(C2015204176) （C2015204176）
