畜牧兽医学报2017，Vol.48Issue(6)：1000-1006,7.DOI:10.11843/j.issn.0366-6964.2017.06.004
Gab1和Sfmbt2基因在成年普通牛8种不同组织中的印记状态分析
Genomic Imprinting Status of Gab1 and Sfmbt2 in Different Tissues of Adult Cattle
摘要
Abstract
In order to reveal the imprinting status of Gab1 (Grb2-associated binding protein 1) and Sfmbt2 (Scm-like with four mbt domains 2) in different tissues and placenta of bovine,in this study,we analyzed the imprinting status of Gab1 and Sfmbt2 in 7 bovine tissues (heart,liver,spleen,lung,kidney,muscle and fat) and placenta by direct sequencing the RT-PCR products based on single nucleotide polymorphisms.The result displayed that Gab1 and Sfmbt2 were all expressed in 7 tissues and placenta.We discovered that Gab1 and Sfmbt2 were bi-allelic expressed by comparing sequence chromatograms of RT-PCR products and genomic PCR products at single nucleotide polymorphisms sites,suggesting that Gab1 and Sfmbt2 were not imprinted in adult cattle tissues and placenta.关键词
Gab1/Sfmbt2/印记状态/SNP/牛Key words
Gab1/Sfmbt2/imprinting status/SNP/cattle分类
农业科技引用本文复制引用
王冠楠,赵宇鹏,陈玮娜,张萃,徐达,李冬杰,李世杰..Gab1和Sfmbt2基因在成年普通牛8种不同组织中的印记状态分析[J].畜牧兽医学报,2017,48(6):1000-1006,7.基金项目
国家自然科学基金(31372312) （31372312）
河北省自然科学基金(C2016204092) （C2016204092）
