畜牧兽医学报2017，Vol.48Issue(6)：1007-1016,10.DOI:10.11843/j.issn.0366-6964.2017.06.005
蒙古马高负荷运动训练前后转录组差异表达分析
Mongolian Horses Transcriptome Differential Expression Analysis before and after a High Load Exercise Training
摘要
Abstract
The aim of this study was to analyze the Mongolian horse transcriptome differential expression before and after a high load exercise training.This research selected 6 Mongolian horses with high load training for 4 months.Muscle samples were collected before and after the training respectively.The transcriptome library was established using the second generation sequencing technology.We enriched the differentially expressed genes to the GO and KEGG pathway,and screened the significantly different metabolic pathways and related genes.1 102 differentially ex pressed genes(DEGs) in transcriptome library were screened out,of which 299 were down-regulated and 803 were up-regulated after high load training,respectively.These DEGs were annotated to 3 398 GO terms,including muscle structure development,cardiovascular system development,circulatory system development,muscle tissue development,muscular organ development,muscle contraction,muscle cell differentiation,etc.Also the DEGs were annotated to 193 KEGG pathways including some athletics related pathways:dilated cardiomyopathy (DCM),hypertrophic cardiomyopathy,cardiac muscle contraction,calcium signaling,the actin cytoskeleton adjustment,etc.Our findings provide a theory support for further investigating the molecular mechanism of Mongolian horse strong stamina and a new research method for Mongolian horses athletic characteristics.关键词
蒙古马/高负荷运动/转录组Key words
Mongolian horse/high load exercise/transcriptome分类
农业科技引用本文复制引用
赵启南,芒来,白东义,赵一萍,李蓓,乌尼尔夫,乌英嘎,苏日嘎,敖乳嘎..蒙古马高负荷运动训练前后转录组差异表达分析[J].畜牧兽医学报,2017,48(6):1007-1016,10.基金项目
国家自然科学基金(31360538 （）
31472070) （）
内蒙古自治区科技厅重点实验室建设项目(20130902) （20130902）
内蒙古自治区高等学校科学研究一般项目(NJZY0701) （NJZY0701）
