畜牧兽医学报2017，Vol.48Issue(6)：1028-1034,7.DOI:10.11843/j.issn.0366-6964.2017.06.007
肾上腺素能受体β2和胆碱能受体M1在小鼠睾丸的表达定位及功能研究
Study on the Expression and Functions of Adrenergic Receptor β2 and Muscarinic Acetylcholine Receptor M1 in Mouse Testis
摘要
Abstract
The experiment was conducted to study the expression and functions of adrenergic receptor β2 (β2AR) and muscarinic acetylcholine receptor M1 (M1R) in mouse testis.The expression of β2AR and M1R mRNA in mouse testis was analysed by method of RT-PCR at different developmental stages.Expression and localization of β2AR and M1R in mouse testis were examined by immunohistochemistry.The mouse Leydig tumor cells-1 (MLTC-1) were cultured in vitro to study the functions of β2AR and M1R.The results showed that β2AR and M1R mRNA obviously expressed in mouse testis at different developing stages.β2AR and M1R mostly localized in Leydig cells of testis.NE and Ach improved the proliferation of MLTC-1 in the concentration of 10-5 mol · L-1 and 10-6mol · L-1(p＜0.05).β2AR blocker butoxamine (Buto) and M1R blocker pirenzepine (Pire) both blocked the effects of NE and Ach(P＜0.05).NE and Ach obviously reduced the expression of 3β-HSD mRNA in MLTC-1,and the β2AR blocker Buto and M1R blocker Pire halted the effects by blocking β2AR and M1R.NE improved the expression of ERα mRNA,but Ach enhanced the expression of ERβ mRNA,receptor blocker Buto and Pire didn't block the effects of NE and Ach.The results indicated that the functions of NE and Ach regulating the proliferation of Leydig cells and synthesis of testosterone were mediated by β2AR and M1R,but the effects of NE and Ach on the expression of ERα and ERβ were not mediated by β2AR and M1R.关键词
肾上腺素能受体/胆碱能受体/受体阻断剂/睾丸Key words
adrenergic receptors/muscarinic acetylcholine receptors/receptor blockers/testis分类
生物科学引用本文复制引用
霍书英,李玉荣,武现军..肾上腺素能受体β2和胆碱能受体M1在小鼠睾丸的表达定位及功能研究[J].畜牧兽医学报,2017,48(6):1028-1034,7.基金项目
河北省教育厅科学研究项目(Z2013021) （Z2013021）
国家自然科学基金(31172094) （31172094）
