畜牧兽医学报2017，Vol.48Issue(6)：1076-1084,9.DOI:10.11843/j.issn.0366-6964.2017.06.012
猪圆环病毒3型检测及其Cap结构序列和抗原性预测分析
Survey on Detection and Analyses of Cap Antigenicity Prediction of Porcine Circovirus Type 3 Isolated from Partial Provinces of Southern China
摘要
Abstract
A novel genotype of porcine circovirus type 3 (PCV3) has recently been found to be widely distributed in several states in America.In order to confirm that whether PCV3 has been distributed in southern China,and make prediction about whether the PCV2 vaccines have protective effect on PCV3.In this study,nested-PCR was used for detection of PCV3 samples collected from partial provinces of southern China,PyMol and web server were used to analyze the structure of the PCV3 capsid protein (Cap).PCV3 was detected in the pigs with PDNS-like syndrome,and these pigs in farms of several provinces of southern China were co-infected with both PCV 2 and PCV 3.Analyses of the primary sequences and the 3-Dimensional (3D) structures of both PCV 2 and PCV 3 Cap demonstrates large,distinct differences in their antigenic epitopes,suggesting cross-protection between PCV2 and PCV3 is impossible.Therefore,the development of novel vaccines will most likely be required to control PCV3 prevalence and infections on farms.关键词
猪圆环病毒3型/皮炎肾病综合征/巢式PCR/核衣壳蛋白质Key words
porcine circovirus type 3 (PCV3)/porcine dermatitis and nephropathy syndrome (PDNS)/nested-PCR/capsid protein分类
农业科技引用本文复制引用
湛洋,王东亮,王乃东,蒋一凡,黄坤,崔尚金,姜平,杨毅..猪圆环病毒3型检测及其Cap结构序列和抗原性预测分析[J].畜牧兽医学报,2017,48(6):1076-1084,9.基金项目
湖南省教育厅重点项目(15A086) （15A086）
湖南省研究生科研创新项目(CX2016B285 （）
CX2016B314) （）
