畜牧与兽医2017，Vol.49Issue(6)：1-8,8.
猪克隆胚早期发育过程中供体线粒体的动态变化及胞吐样排出对其清除的影响
Dynamic changes of donor mitochondria during the early development of porcine cloned embryos and the effect of cytoplasmic discharge on their clearance
摘要
Abstract
To observe the dynamic distribution of donor mitochondria in cloned embryos during their early development and provide the preliminary inquisition to the specificity of cytoplasmic discharge on the clearance of donor mitochondria,cloned embryos were produced by using pig fetal fibroblasts with pre-labeled mitochondria as donor cells while parthenogenetic activation (PA) embryos were used as control.The immunostaining with the confocal microscopy and transmission electron microscopy observation were performed on the embryos which were at different developmental stages.Our results showed that donor mitochondria were gradually scattered to part of the cytoplasm (4 h) from perinuclear area (0 h) after electric activation.Compared with PA embryos,mitochondria of cloned embryos had more abnormal morphology.No matter at 2-cell (28 h) or 4-cell (52 h) stages,we observed 3 distribution forms of donor mitochondria.At 2-cell stage of pig cloned embryos,donor mitochondria were not observed in both blastomeres of 21.95％ cloned embryos,and 26.83％ cloned embryos had donor mitochondria in one blastomere,while 51.22％ cloned embryos still had donor mitochondria in both two blastomeres.Even to the blastocyst stage,donor mitochondria still could be observed in few cloned blastocysts.In addition,about 87.93％ 2-cell embryos showed "redundant" cytoplasm between the blastomeres,and donor mitochondria could be observed in this area of certain such embryos.The addition of 5 μg/mL cytochalasin B (CB) did not significantly change the proportion of cloned embryos which owned donor mitochondria.Meanwhile,a similar phenomenon of cytoplasmic discharge was noticed in 2-cell PA embryos,and the maternal mitochondria could be also observed in the discharge area.Our results suggested that donor mitochondria distributed unequally in 2-cell and 4-cell cloned embryos,and could be extruded with cytoplasmic discharge in some 2-cell cloned embryos,but the discharge was not specific for the removal of donor mitochondria.关键词
猪/克隆胚/供体线粒体/清除/胞质排出Key words
pig/cloned embryo/donor mitochondria/clearance/cytoplasmic discharge分类
农业科技引用本文复制引用
迟大明,曾亚琼,徐明珠,汪成飞,牛英杰,刘红林,李娟..猪克隆胚早期发育过程中供体线粒体的动态变化及胞吐样排出对其清除的影响[J].畜牧与兽医,2017,49(6):1-8,8.基金项目
国家自然科学基金(31402076) （31402076）
淮安市产学研协同创新计划(HAC2014018) （HAC2014018）
