畜牧与兽医2017，Vol.49Issue(6)：15-19,5.
麒麟鸡MHC-B区域微卫星位点LEI0258遗传多样性研究
Genetic diversity of Frizzle chicken estimated by the micro-satellite LEI0258 in chicken MHC-B region
摘要
Abstract
To elucidate polymorphism and evolution of Frizzle chicken,the tandem repeat LEI0258 located within the B region of chicken major histocompatibility complex (MHC-B region) was applied.Forty samples of Frizzle chicken were used to genotype and sequence for the analysis of allele and nucleotide polymorphism and median-joining network.A total of 12 alleles ranging from 193 bp to 489 bp in 40 samples were found,of which six was novel for Frizzle chicken.The microsatellite LEI0258 showed high levels of polymorphism in this breed,with 0.800,0.840 and 0.811 for observed heterozygosity,expected heterozygosity and polymorphic information content,respectively.Five alleles were corresponding to 11 available serotypes.Our results,incorporating with published alleles,were classified into five clusters,based on the SNPs and indels located the sequences flanking the repeats.Frizzle chicken existed in all five clades,and red jungle fowl was also found in the clades A,B and E.Our results suggested that Frizzle chicken maintained high levels of genetic polymorphism in LEI0258,and were origin from red junglefowl with multiple domestication.关键词
麒麟鸡/主要组织相容性复合体/LEI0258/多态性/进化/血清型Key words
Frizzle chicken/major histocompatibility complex/LEI0258/polymorphism/evolution/serotype分类
农业科技引用本文复制引用
黄勋和,邓春苗,余哲琪,李威娜,陈洁波,钟福生..麒麟鸡MHC-B区域微卫星位点LEI0258遗传多样性研究[J].畜牧与兽医,2017,49(6):15-19,5.基金项目
广东省自然科学基金项目(2014A030307018) （2014A030307018）
嘉应学院“创新强校工程”项目(CQX019) （CQX019）
广东省公益研究与能力建设项目(2015A020208020 （）
2016A030303068) （）
嘉应学院重点科技计划项目(2015KJM03) （2015KJM03）
