畜牧与兽医2017，Vol.49Issue(6)：77-82,6.
Kisspeptin-10对热应激引起大鼠心肌损伤的影响
Effects of kisspeptin-10 on myocardial injury induced by heat stress in rats
摘要
Abstract
The aim of our present study was to investigate the effects of kisspeptin-10 on the heat stress induced myocardial injury in SD rats.Twenty-four male Sprague-Dawley rats were randomly assigned into three groups:control group (C),heat stress group (H) and hypodermic injection of kisspeptin-10 after heat stress (KH).After being exposed to different treatments,histomorphology of heart tissues in three groups were observed through hematoxylin eosin (HE) staining,masson staining and transmission electron microscope (TEM).Then,the mRNA expression of cardiomyopathy genes including ITGA4,ITGB8,BNP,MYL7,HIF-1o and ITGB7 were tested by RT-PCR.The key protein ITGA4,ITGB8 and BNP were involved in many heart diseases pathways and were detected by Western-blot.The results showed that there was a degree of myocardial damage in heat stress group compared to control.However,heat stress group with kisspeptin-10 treatment demonstrated a relief symptoms compared to heat stress group (more regularly arranged cardiomyocytes,more evenly stained nucleus,obviously decreased proliferation of fiber and injured mitochondria).Furthermore,the genes including ITGB8 and BNP in group H were alterated at the transcription and translation level but tend to be normal in group KH.The effects of kisspeptin-10 on myocardial injury induced by heat stress in rats would provide the theoretical basis to the deeper research.关键词
kisspeptin-10/SD大鼠/热应激/ITGB8/BNP/心肌损伤Key words
kisspeptin-10/SD rats/heat stress/ITGA4/ITGB8/myocardial injury分类
农业科技引用本文复制引用
张莹,马志禹,索川,侯园龙,张征,贾翠翠,李翔,雷治海,苏娟..Kisspeptin-10对热应激引起大鼠心肌损伤的影响[J].畜牧与兽医,2017,49(6):77-82,6.基金项目
中央高校基本业务费自主创新重点项目(KYZ201526) （KYZ201526）
