畜牧与兽医2017，Vol.49Issue(6)：125-129,5.
锦鲤疱疹病毒GY1506株的分离鉴定
Isolation and identification of koi herpesvirus GY1506
摘要
Abstract
One strain of koi herpesvirus (KHV) was isolated and identified from diseased common carp from a fish farm in Jiangsu.The diseased cyprinoid manifested with typical clinical signs including enophthalmia (sunken eyes) and haemorrhages on the skin and base of fins,and fin erosion.The postmortem examination revealed the intestines hyperemia and liver,kidney,cholecyst were obviously swollen.The CCB cells were inoculated with supernatant of the diseased fish tissues and 14 days later,typical cell pathological changes could be observed,such as roundout and exfoliation.Positive results were obtained from all detected gill and intestine samples according to a standard PCR diagnosis of CyHV-3 proposed by Office International des Epizooties (OIE).Homology analysis of nucleotide sequences showed that the thymidine kinase (TK) gene from infected cyprinoid shared 100％ sequence identity to that of the KHV-J.Based on the TK gene sequences,phylogenetic tree was constructed and the result indicated that the viral strain in our study belongs to Asian genotype,named KHV-GY1506.关键词
鲤鱼/锦鲤疱疹病毒/TK基因/病毒分离Key words
cyprinoid/koi herpervirus/TK gene/virus isolation分类
农业科技引用本文复制引用
王凤芝,丁超,曹洁,徐晔,段宏安,周毅,李金华,程积进..锦鲤疱疹病毒GY1506株的分离鉴定[J].畜牧与兽医,2017,49(6):125-129,5.基金项目
国家质检总局公益性项目(201310221) （201310221）
连云港市科技公共服务平台项目(JC1404) （JC1404）
