西南国防医药2017，Vol.27Issue(6)：541-544,4.DOI:10.3969/j.issn.1004-0188.2017.06.001
p65通过调节GLUT3基因的表达抑制胶质瘤细胞增殖和侵袭能力
Effect of p65 on invasion and proliferation ability of human glioblastomas cells through GLUT-3
摘要
Abstract
Objective To investigate the effect and the related mechanism of p65 on the invasion and proliferation ability of human ghoblastomas U87 cells.Methods p65 siRNA was used to silence the expression of p65.pIRES2-ZsGreen1-p65 was used to overexpression the expression of p65.Expression of GLUT-3 was detected by Western blotting;Effect of p65 on the invasion ability of U87 cells was detected by Transwell matrigel invasion assays;Effect of p65 on the proliferation ability of U87 cells was detected by CCK-8.Results Knockdown or overexpression the expression of p65 could reduce or increase the expression of GLUT-3.Knockdown or overexpression the expression of p65 could inhibit or promote the invasion ability of human glioblastomas U87 cells.Knockdown or overexpression the expression of p65 could inhibit or promote the invasion and proliferation ability of human glioblastomas U87 cells.Conclusion The expression of p65 could regulate the invasion and proliferation ability of human glioblastomas U87 cells through regulating the expression of GLUT-3.关键词
GLUT-3/p65/胶质瘤/NF-κB/侵袭/迁移Key words
GLUT-3/p65/glioma/proliferation/invasion/migration分类
医药卫生引用本文复制引用
杨堃,郑传宜,李争争,张家润,叶富跃,吴然..p65通过调节GLUT3基因的表达抑制胶质瘤细胞增殖和侵袭能力[J].西南国防医药,2017,27(6):541-544,4.基金项目
国家自然科学基金资助课题(81260371) （81260371）
