眼科新进展2017，Vol.37Issue(6)：506-510,5.DOI:10.13389/j.cnki.rao.2017.0128
白血病抑制因子(LIF)对视网膜视锥细胞氧化损伤的保护作用及机制
Protective effects and mechanisms of LIF on cone photoreceptors from oxidant injury
摘要
Abstract
Objective To investigate the role of leukemia inhibitory factor (LIF) in cell survival of cone photoreceptors and the underlying mechanism following oxidant injury.Methods 661W cells were cultured and randomly divided into normal control group,LIF intervention group,H2O2 intervention group,S3 I201 intervention group,H2O2 + LIF intervention group,H2O2 + S3I201 intervention group and H2O2 + LIF + S3I201 intervention group,according to the different intervention drugs.MTT assay and Western blot were used to detect the effects of LIF pretreatment on 661W activity and the expression of STAT3 protein and its phosphorylation level after oxidative damage.Using STAT3-specific inhibitor S3I201 to block the STAT3 signal transduction pathway,MTT assay and real-time PCR were used to detect the effects of STAT3 signaling pathway on 661 W activity and the mRNA expression of survivin bcl-2 and bcl-xi after oxidative damage.Results Compared to H2O2 intervention group,the relative protein expression of p-Tyr705-STAT3 was increased in H2O2 + LIF intervention group,the difference was statistically significant (P ＜ O.05);The relative protein expression of p-Tyr705-STAT3 was decreased in H2O2 + S3I201 intervention group,the difference was statistically significant (P ＜ 0.05).Compared to H2O2 intervention group,the cell activity of 661W cells was increased in H2O2 + LIF intervention group,the difference was statistically significant(P ＜0.05).Compared to H2O2 + LIF intervention group,the cell activity of 661W cells was decreased in H2O2 + LIF + S3I201 intervention group,the difference was statistically significant (P ＜ 0.05).Compared to normal control group,the mRNA expression of bcl-2 and bcl-xl were increased in LIF intervention group,H2O2 intervention group and H2O2 + LIF intervention group,the differences were statistically significant (all P ＜ 0.05).Compared to H2O2 + LIF intervention group,the mRNA expression of bcl-2 and bcl-xl were decreased in H2O2 + LIF + S3I201 intervention group,the differences were statistically significant (all P ＜ 0.05).Conclusion LIF has protective effect on oxidative injury of cone photoreceptors,and it may contribute to cell survival through activation of the STAT3 signaling transduction pathway and its related survivin.关键词
氧化损伤/白血病抑制因子/视锥细胞Key words
oxidative injury/leukemia inhibitory factor/cone photoreceptor分类
医药卫生引用本文复制引用
董淑倩,李秋明,刘双珍..白血病抑制因子(LIF)对视网膜视锥细胞氧化损伤的保护作用及机制[J].眼科新进展,2017,37(6):506-510,5.基金项目
国家自然科学基金联合基金项目(编号:U1304812) （编号:U1304812）
河南省医学科技攻关计划普通项目(编号:201602080) （编号:201602080）
Joint Project of National Natural Science Foundation of China (No:U1304812) （No:U1304812）
Medical Science and Technology Project of Henan Province (No:201602080) （No:201602080）
