眼科新进展2017，Vol.37Issue(6)：511-514,4.DOI:10.13389/j.cnki.rao.2017.0129
脂联素对RF/6A细胞增殖、迁移及管腔形成的影响
Effects of adiponectin on proliferation, migration and tube formation of RF/6A cells
摘要
Abstract
Objective To investigate the effects of adiponectin (APN) on proliferation,migration and tube formation of RF/6A cells,and explore the effects of APN on choroidal and retinal angiogenesis.Methods Well cultured RF/6A cells were randomly divided into the control group and three groups with different concentrations of recombinant adiponectin (5 pg · mL-1,50 pg · mL-1,500 pg · mL-1) for 1 hour.After 24 hours,cell proliferation,migration and tube formation were detected by MTT assay,wound scratch assay and seeding cells in matrigel,respectively.Results The cell proliferation in all APN groups was weaker than that of control group (P ＜ 0.05),the cell migration area (pixels) of all APN groups was significantly smaller than that of the control group (P ＜ 0.05),and the number of tube formation of all APN groups was significantly less than the control group (P ＜ 0.05).Cell proliferation,migration and tube formation decreased along with the increase of APN concentration.Conclusion APN can obviously inhibit the angiogenesis process of RF/6A cells.This inhibitive effect indicates the protective role of APN in choroidal and retinal angiogenesis.关键词
脂联素/血管生成/脉络膜新生血管/视网膜新生血管Key words
adiponectin/angiogenesis/choridal neovascularization/retinal neovascularization分类
医药卫生引用本文复制引用
谢坤鹏,王新,杜军辉,刘平..脂联素对RF/6A细胞增殖、迁移及管腔形成的影响[J].眼科新进展,2017,37(6):511-514,4.基金项目
陕西省自然科学基础研究计划面上项目(编号:2016JM8018) （编号:2016JM8018）
National Natural Science Foundation of Shannxi Province(No:2016JM8018) （No:2016JM8018）
