眼科新进展2017，Vol.37Issue(6)：515-518,4.DOI:10.13389/j.cnki.rao.2017.0130
硫化氢对H2O2诱导的视网膜神经节细胞氧化损伤的保护作用
Protective effects of H2S against H2O2-induced oxidative injury in RGC
摘要
Abstract
Objective To investigate the protective effects and possible mechanism of hydrogen sulfide (H2S) against oxidative stress injury induced by hydrogen peroxide (H2O2) in retinal ganglion cell-5 (RGC-5).Methods RGC-5 cells were divided into four groups:RGC-5 group (normal control group),RGC-5 + H2O2 (RGC-5 were cultured in 500 μmol · L-1 H2O2 for 24 hours) group,RGC-5 + NaHS (RGC-5 were cultured in 50 μmol · L-1 NaHS for 30 minutes) + H2O2 (RGC-5 were cultured in 500 μmol · L-1 H2 O2 for 24 hours) group,and RGC-5 + NaHS (RGC-5 were cultured in 50 μmol · L-1 NaHS for 30 minutes) group.Western blots were applied to measure the expression of cytochrome c (Cyt.c) and optic atrophy 1 (OPA1).The fluorescent dye JC-1 assay was chosen to detect the mitochondrial membrane potential (△Ψm).Furthermore,transmission electron microscope was used to observe the morphology of mitochondria.Results Compared with RGC-5 group,the expression of Cyt.c in RGC-5 + H2O2 group decreased in mitochondria,and increased in cytoplasm (all P ＜ 0.05),but there was no statistical difference between RGC-5 group and RGC-5 + NarHS + H2O2 group (all P ＞0.05).Compared with RGC-5 group,the expression of Cyt.c in RGC-5 + NaHS group increased in mitochondria,and decreased in cytoplasm (all P ＜ 0.05).Compared with RGC-5 group,the expression of OPA1 in RGC-5 + H2O2 group decreased in mitochondria,and increased in cytoplasm (all P ＜ 0.05).In RGC-5 + NaHs + H2O2 group and RGC-5 + NaHS group,the expression of OPA1 within and outside the mitochondria had no significant difference compared with RGC-5 group (all P ＞ 0.05).Compared with other three groups,the mitochondrial membrane potential in RGC-5 + H2O2 group obviously decreased,but there was no statistical difference among other three groups (P ＞ 0.05).The mitochondria were globular swelled in RGC-5 group,but in other three groups,the mitochondria had slightly swelled.Conclusion H2S can protect the mitochondrial morphology and functions of RGC? 5 from H2O2-induced oxidative stress via preventing OPA1 release from mitochondria.关键词
氧化应激/视网膜神经节细胞/硫化氢/视神经萎缩蛋白1Key words
oxidative stress/retinal ganglion cell/hydrogen sulfide/optic atrophy 1分类
医药卫生引用本文复制引用
罗鑫,刘太祥,任荟璇,罗艳..硫化氢对H2O2诱导的视网膜神经节细胞氧化损伤的保护作用[J].眼科新进展,2017,37(6):515-518,4.基金项目
贵州省科学技术基金资助(编号:20147588) （编号:20147588）
遵义医学院博士启动基金(编号:201307) （编号:201307）
Guizhou Science and Technology Foundation (No:20147588) （No:20147588）
Doctor's Initial Funding of Zunyi Medical College (No:201307) （No:201307）
