眼科新进展2017，Vol.37Issue(6)：519-522,4.DOI:10.13389/j.cnki.rao.2017.0131
偶联穿膜肽携带外源蛋白进入眼球的研究
Study on cell-penetrating peptides carrying foreign proteins into eyeball
摘要
Abstract
Objective To investigate whether poly arginine as the carrier can carry foreign proteins to penetrate the cell membrane and even penetrate the eyeball barrier.Methods Poly-Args (R9) was used as a CPP in this study.R9-green fluorescent protein (GFP) and GFP were constructed.In vitro,human lens epithelial cells were treated with these two proteins.Then,MTT assay were used to detect whether the protein could affect the proliferation of the cells.Flow cytometry and laser confocal microscopy were used to detect the penetrability of CPPs on the cells.In vivo,eyes of mice were treated with protein in eye drops way for 7 days.Then total protein were extracted,ELISA were used to detect the penetrability of CPPs.Results The results of MTT,flow cytometry and laser confocal microscopy showed that CPPs could carry protein into cells in a dose dependent manner without affecting cell proliferation.In vivo,slit lamp showed that the mice eyeballs had no any abnormal after treated by GFP,R9-GFP,and ELISA results also showed that R9 could effectively get foreign protein into the eyeball.Conelusion R9 can carry foreign protein into the cell membrane and eyeball barrier.This study provides the basis for the eye medication and dosing mode improvement.关键词
细胞穿膜肽/9聚精氨酸/眼球屏障/晶状体上皮细胞Key words
cell-penetrating peptides/Poly-arginine/eyeball barrier, lens epithelial cell分类
医药卫生引用本文复制引用
赵婷婷,牛晓霞,车庭华,苏大迎,彭绍民..偶联穿膜肽携带外源蛋白进入眼球的研究[J].眼科新进展,2017,37(6):519-522,4.基金项目
黑龙江省卫生计生委科研课题资助(编号:2014-044) （编号:2014-044）
Foundation of Health and Family Planning Commission of Heilongjiang Province (No:2014-044) （No:2014-044）
