眼科新进展2017，Vol.37Issue(6)：523-526,4.DOI:10.13389/j.cnki.rao.2017.0132
青蒿琥酯对人Tenon囊成纤维细胞增殖与凋亡的影响
Effects of artesunate on cell proliferation and apoptosis of human Tenon's capsule fibroblasts
陈静 1陈艺 2邹秀兰 1邹玉平1
作者信息
- 1. 510010 广东省广州市,广州军区广州总医院眼科
- 2. 510010 广东省广州市,广州军区广州总医院培训中心
- 折叠
摘要
Abstract
Objective To investigate the effects of artesunate (Art) on cell proliferation and apoptosis of human Tenon's capsule fibroblasts (HTFs),and discuss the countermeasures of bleb scarfing in glaucoma.Methods In vitro,HTFs were cultivated and applicated by different concentrations (50 μg · mL-1,100 μg · mL-1,150 μg ·mL-1,200 μg · mL-1) of Art for 48 hours.The effect of Art on cell proliferation was assessed by MTT method.The rate of apoptosis induced by Art was determined by flow cytometry.Western Blot was performed to detect the relative expression levels of Bax and Bcl-2 after Art was treated.Results After treated with Art for 48 hours,compared with blank control group,Art (50 μg · mL-1,100 μg · mL-1,150 μg · mL-1,200 μg · mL-1) group exhibited notable anti-proliferative effect on HTFs with concentration-dependence (all P ＜ 0.05).The results of flow cytometry showed that the apoptosis rates (8.80％ ±0.88％,11.60％ ±0.56％,16.30％ ±1.03％,23.40％ ±1.62％) of HTFs were significantly enhanced with the increase of Art concentration (all P ＜ 0.05).The relative expression levels of Bax were obviously high with the increase of Art concentration,while Bcl-2 levels were significantly low with the increase of Art concentration (all P ＜ 0.05).Conclusion Art can inhibit the proliferation and induce cell apoptosis of HTFs possibly by enhancing the expression of Bax and reducing the expression of Bcl2.Art may be a potential drug in preventing fibrous scar formation after glaucoma filtration surgery.关键词
青蒿琥酯/人Tenon囊成纤维细胞/细胞增殖/细胞凋亡/青光眼Key words
artesunate/human Tenon's capsule fibroblasts/cell proliferation/cell apoptosis/glaucoma分类
医药卫生引用本文复制引用
陈静,陈艺,邹秀兰,邹玉平..青蒿琥酯对人Tenon囊成纤维细胞增殖与凋亡的影响[J].眼科新进展,2017,37(6):523-526,4.
