眼科新进展2017，Vol.37Issue(6)：527-530,547,5.DOI:10.13389/j.cnki.rao.2017.0133
白皮杉醇对青光眼大鼠视网膜神经节细胞的保护作用
Protective effects of piceatannol on retinal ganglion cells in experimental glaucoma rats
杜倩 1侯旭 2李剑波 1周蜂人 1杜琛3
作者信息
- 1. 473000 河南省南阳市,南阳市中心医院眼科
- 2. 710032 陕西省西安市,第四军医大学西京医院眼科
- 3. 411300 河南省南阳市,南阳市唐河县城郊医院公共卫生服务科
- 折叠
摘要
Abstract
Objective To investigate the protective effects of piceatannol on retinal ganglion cells (RGCs) in rats with glaucoma.Methods Forty rats were randomly divided into 4 groups:control group,model group,low dose piceatannol treatment group and high dose piceatannol treatment group.Photocoagulation method was used to establish the experimental glaucoma model in rats,and then rats were given 100 mg· kg-1 or 200 mg · kg-1 of piceatannol by gavage.The intraocular pressure was measured before and after the model was established.Rat retinal ganglion cells were labeled and counted using FG staining.Retinal tissue pathological morphology was observed by HE staining.The protein expression of p-JNK,p-c-Jun,p-ERK,p-p38 MAPK and TNF-α were measured by western blot.Results Compared with control group,the intraocular pressure,the protein expressions of p-JNK,p-c-Jun p-ERK,p-p38 MAPK and TNF-α were significantly increased in model group (all P ＜ 0.05).However,the number of RGCs were lower in model group(P ＜0.05).Furthermore,there were cavitation and edema changes in retinal tissue of model group.Compared with model group,piceatannol treatment markediy increased the number of RGCs(P =0.003,0.002),improved the pathological morphology of retinal tissue,and reduced the protein expressions of p-JNK,p-c-Jun p-ERK,p-p38 MAPK and TNF-α (all P ＜ 0.05),especially for the high concentration.Conclusion Piceatannol can protect against RGCs injury in glaucoma rats,and the mechanism may be associated with inhibition of MARK signaling pathway.关键词
白皮杉醇/视网膜神经节细胞/MARK信号通路Key words
piceatannol/retinal ganglion cells/MARK signaling pathway分类
医药卫生引用本文复制引用
杜倩,侯旭,李剑波,周蜂人,杜琛..白皮杉醇对青光眼大鼠视网膜神经节细胞的保护作用[J].眼科新进展,2017,37(6):527-530,547,5.
