湖北医药学院学报2017，Vol.36Issue(2)：120-123,128,5.DOI:10.13819/j.issn.1006-9674.2017.02.006
紫草宁通过PI3K/Akt通路抑制百草枯诱导的SH-SY5Y细胞损伤
Shikonin Inhibits Paraquat-induced SH-SY5Y Cell Injury through PI3K/Akt Activation
摘要
Abstract
Objective To explore the effect of and mechanism of shikonin on SH-SY5Y cells derived from paraquat-induced Parkinson's disease (PD) model.Methods SH-SY5Y cells were pretreated with Shikonin for 1 hour and then treated with paraquat (1 mmol / L) and/or LY294002 (1 μmol/L) for 24 h.Then cell viability and p-Akt (ser 473) level were measured by MTT and western blotting respectively.Rtesults Middle and high concentration of shikonin decreased paraquat-induced SH-SY5Y cell death and increased the level of p-Akt.LY294002 could reduce shikonin-induced p-Akt elevation and attenuate the effect of shikonin on the cell death rate in the injury model Conclusion A certain concentration of shikonin could inhibit paraquat-induced oxidative stress injury through the activation of PI3K/Akt pathway.关键词
紫草宁/百草枯/帕金森/氧化应激/SH-SY5Y/AktKey words
Shikonin/Paraquat/Parkinson's disease/Oxidative stress/SH-SY5Y/Akt引用本文复制引用
吕艳霞,赵丹,张志锋..紫草宁通过PI3K/Akt通路抑制百草枯诱导的SH-SY5Y细胞损伤[J].湖北医药学院学报,2017,36(2):120-123,128,5.基金项目
湖北省教育厅科学研究计划指导性项目(B2015492) （B2015492）
十堰市科学技术研究与开发项目(16Y07) （16Y07）
湖北医药学院研究生启动金(2010QDJ13) （2010QDJ13）
