中国当代儿科杂志2017，Vol.19Issue(7)：820-825,6.DOI:10.7499/j.issn.1008-8830.2017.07.018
膜联蛋白A2对肺炎支原体诱导的人气道上皮细胞EGFR/NF-κB信号转导及黏蛋白表达的影响
Effect of annexin A2 on EGFR/NF-κB signal transduction and mucin expression in human airway epithelial cells treated with Mycoplasma pneumoniae
申冬冬 1袁飞 1侯江红1
作者信息
- 1. 河南省中医院儿科,河南 郑州 450002
- 折叠
摘要
Abstract
Objective To investigate the effect of annexin A2 (AnxA2) on epithelial growth factor receptor (EGFR)/nuclear factor-κB (NF-κB) signal transduction and mucin expression in human airway epithelial H292 cells treated with Mycoplasma pneumoniae (MP). Methods H292 cells were divided into control group, MP group, NC-siRNA+MP group, and AnxA2 siRNA+MP group. The cells in the MP group were incubated with 5 μg/mL MP antigen for 2 hours. The cells in the NC-siRNA+MP and AnxA2 siRNA+MP groups were transfected with NC-siRNA and AnxA2 siRNA for 24 hours, followed by MP antigen stimulation for 2 hours. The MTT method was used to measure cell viability;quantitative real-time PCR was used to measure the mRNA expression of AnxA2; Western blot was used to measure the protein expression of AnxA2, phosphorylated EGFR (p-EGFR), and phosphorylated p65 NF-κB (p-p65 NF-κB);ELISA was used to measure the secretion of mucin 5AC (MUC5AC) and mucin 5B (MUC5B). Results The MP and NC-siRNA+MP groups had lower cell viability than the control group (P<0.05). The AnxA2 siRNA+MP group had higher cell viability than the MP and NC-siRNA+MP groups and lower cell viability than the control group (P<0.05). The MP and NC-siRNA+MP groups had significantly higher mRNA and protein expression of AnxA2 than the AnxA2 siRNA+MP group (P<0.05). Compared with the control group, the MP and NC-siRNA+MP groups had significant increases in the protein expression of p-EGFR, p-p65 NF-κB, MUC5AC, and MUC5B (P<0.05); the AnxA2 siRNA+MP group had lower protein expression than the MP and NC-siRNA+MP groups, but higher protein expression than the control group (P<0.05). Conclusions AnxA2 is involved in the airway lesion induced by MP antigen via mediating EGFR/NF-κB signaling activation and mucin expression in human airway epithelial cells.关键词
膜联蛋白A2/肺炎支原体/表皮生长因子受体/核因子κB/气道上皮细胞Key words
Annexin A2/Mycoplasma pneumoniae/Epidermal growth factor receptor/Nuclear factor κB/Airway epithelial cell引用本文复制引用
申冬冬,袁飞,侯江红..膜联蛋白A2对肺炎支原体诱导的人气道上皮细胞EGFR/NF-κB信号转导及黏蛋白表达的影响[J].中国当代儿科杂志,2017,19(7):820-825,6.
