中国动脉硬化杂志2017，Vol.25Issue(6)：548-552,5.
普罗布考对炎性单核细胞亚群分化的影响及可能机制
The effect of probucol on the differentiation of inflammatory monocytes and its potential mechanism
摘要
Abstract
Aim To investigate the effect of probucol on the differentiation of inflammatory monocyte subsets and its potential mechanism.Methods The primary single cell suspension of spleen was prepared from 8 week old C57BL/6 mice.The differentiation of monocyte subsets was induced by 100 kU/L recombinant interferon-γ,then intervened with 25,50,100 μmol/L probucol,in order to observe their effects on the differentiation of monocytes.The cells were stained with anti-CD11b-PE and anti-Ly-6C-FITC antibody,and the differentiation of monocyte subsets was detected by flow cytometry,then the data were analyzed by Flowjo software.Using intracellular cytokine staining and 2',7'-dichlorofluorescein-diacetate probe,the level of reactive oxygen species (ROS) in monocyte subsets was detected by flow cytometry.NADPH oxidase activity in human monocytic cell line THP-1 was detected by using NADPH oxidase test kit.Results In a concentration dependent manner,25,50,100 μmol/L probucol inhibited the differentiation of Ly-6Chi inflammatory monocyte subsets in the spleen primary cells.The level of ROS in Ly-6C+ inflammatory monocyte subset was 2 times of that in Ly-6C-inflammatory monocyte subset.At the same time,25,50,100 μmol/L probucol inhibited the production of ROS in Ly-6C+ inflammatory monocyte subset in a concentration dependent manner.100 μmol/L probucol significantly inhibited the activity of NADPH oxidase in THP-1 cells.Conclusion Probucol may interfere with the activity of NADPH oxidase,thereby inhibiting the differentiation of Ly-6Chi inflammatory monocyte subset and the production of ROS.关键词
单核细胞/异质性/普罗布考/氧化应激/NADPH氧化酶Key words
Monocyte/Heterogeneity/Probucol/Oxidative stress/NADPH oxidase分类
医药卫生引用本文复制引用
张敏丽,侯禹晨,张大庆..普罗布考对炎性单核细胞亚群分化的影响及可能机制[J].中国动脉硬化杂志,2017,25(6):548-552,5.基金项目
国家自然科学基金项目(81200199) （81200199）
