中国动脉硬化杂志2017，Vol.25Issue(6)：553-558,6.
MicroRNA-155通过CEH影响巨噬细胞泡沫化过程
MicroRNA-155 affects macrophage foaming through CEH
摘要
Abstract
Aim To study the mechanism of microRNA-155(miR-155) depending on cholesteryl ester hydrolase (CEH) to influence foam cells formation.Methods Oil red O staining and HPLC were used to analyze cholesteryl ester content,and the positive cells and cholesterol ester content were observed.Western-blot was used to the expression of CEH,ATP binding cassette transporter A1(ABCA1),scavenger receptor AⅠ(SR-A).Results The positive cell ratio and the cholesterol efflux were dramatically decreased by miR-155 mimics(P＜0.05);the positive cell ratio,the cholesterol efflux,the expression of SR-A were significantly decreased (P＜ 0.05),while the expression of CEH and ABCA1 were significantly increased in miR-155 mimics group respectively(P＜0.05).The expression of SR-A,the positive cell ratio,the cholesterol efflux were significantly increased(P＜0.05),while the expression of CEH and ABCA1 were significantly decreased in microRNA-155+siRNA-CEH group and siRNA-CEH group respectively(P＜0.05).Conclusion The high expression of microRNA-155 can inhibit the formation of foam macrophages,which may be enhanced by CEH affecting the expression of ABCA1 and SR-A.关键词
MicroRNA-155/胆固醇酯水解酶/巨噬细胞Key words
MicroRNA-155/Cholesterol ester hydrolase/Macrophages分类
医药卫生引用本文复制引用
张凤香,李晨光,孙大鹏..MicroRNA-155通过CEH影响巨噬细胞泡沫化过程[J].中国动脉硬化杂志,2017,25(6):553-558,6.基金项目
国家自然科学基金(81541099) （81541099）
辽宁省自然科学基金(2016010330-301) （2016010330-301）
