中国动脉硬化杂志2017，Vol.25Issue(6)：566-570,5.
载脂蛋白A Ⅰ通过JAK2/STAT3信号通路抑制肿瘤坏死因子α的转录表达
Apolipoprotein A Ⅰ inhibit tumor necrosis factor α transcription and expression through JAK2/STAT3 signaling pathway
摘要
Abstract
Aim To explore the effects and mechanism of ApoAⅠ on TNF-α expression in macrophages and plasma TNF-α levels from atherosclerotic mice.Methods Male ApoE-/-mice were fed with high cholesterol diet for 12 weeks and then randomly divided into control group,ApoAⅠ group and ApoAⅠ+AG490(a specific JAK2 inhibitor)group;each group were separately received treatment with PBS,ApoAⅠ (40 μg/g),ApoAⅠ (40μ,g/g) +AG490(4 μg/g) on the third and the first day before sacrifice,12 h before sacrifice all groups were administered with LPS via intraperitoneal injection,TNF-α levels in plasma were measured by ELISA.THP-1 macrophage-derived foam cells were randomly divided into control group,ApoAⅠ(10 mg/L) group and ApoAI(10 mg/L)+AG490(25 μmol/L) group;all groups incubated with LPS (10 μg/L).TNF-α level in supernate were measured by ELISA and TNF-α mRNA expression were examined by RT-PCR.Results Compared with the control group,ApoAⅠ can significandy decrease TNF-α levels in mice plasma,after administered with AG490 ApoAⅠ-inhibition of TNF-α secretion induced by LPS markedly weakened(P＜0.05).In vitro study demonstrated ApoAⅠ can inhibit TNF-α transcription and expression induced by LPS (P＜0.01),AG490 abrogated the anti-inflammatory effect of ApoAⅠ(P＜0.05).Conclusion ApoA Ⅰ inhibited TNF-α transcription and expression through JAK2/STAT3 signaling pathway.关键词
载脂蛋白A Ⅰ/JAK2/STAT3信号通路/肿瘤坏死因子α/转录表达Key words
Apolipoprotein A Ⅰ/JAK2/STAT3 signaling pathway/Tumor necrosis factor α/Transcription and expression分类
医药卫生引用本文复制引用
尹建国,张社兵,彭道泉..载脂蛋白A Ⅰ通过JAK2/STAT3信号通路抑制肿瘤坏死因子α的转录表达[J].中国动脉硬化杂志,2017,25(6):566-570,5.基金项目
国家自然科学基金资助项目(81370393) （81370393）
