中国动脉硬化杂志2017，Vol.25Issue(6)：571-575,5.
丹参乙酸镁抗大鼠心肌缺血再灌注细胞程序性坏死的作用及机制
The effect of salvia magnesium lithospermate B on myocardial ischemia-reperfusion-induced programmed necrosis in rat and its mechanisms
摘要
Abstract
Aim To explore the effect of salvia magnesium lithospermate B (MLB) on myocardial ischemiareperfusion (IR)-induced cells programmed necrosis and its mechanism.Methods Myocardial infarction model was established by occlusion of the left anterior descending coronary artery in rats.After ischemia 1 h and reperfusion for 3 h,myocardial IR injury model was established.Rats were randomly divided into 7 groups:normal control group,sham operation group,IR group,MLB low dose (10 mg/kg) +IR group,MLB high dose (30 mg/kg) +IR group,necrostatin-1 (Nec1;3 mg/kg) +IR group and solvent (normal saline) +IR group (n =6 ～ 8).Myocardial infarct area was detected by triphenyltetrazolium chloride staining.HE staining was used to observe the morphological changes of myocardial tissue.Serum creatine kinase (CK) activity was measured by spectrophotometry.The expression levels of programmed necrosis associated protein receptor interacting protein kinase 1 (RIPK1) and RIPK3 were detected by Western blot.Results High dose of MLB could significantly reduce the myocardial infarct size and CK release,and improve the myocardial tissue structure of IR rats,with the inhibition of RIPK1 and RIPK3 protein expression,which were better than the positive control drug Nec-1.Conclusion MLB has the function of resisting myocardial cell programmed necrosis,and its mechanism is related to the inhibition of RIPK1 and RIPK3 protein expressions.关键词
丹参乙酸镁/缺血再灌注/心肌/程序性坏死/受体相互作用蛋白激酶Key words
Salvia magnesium lithospermate B/Ischemia-reperfusion/Myocardium/Programmed necrosis/Receptor interacting protein kinase分类
医药卫生引用本文复制引用
王玉霞,佘浪,罗秀菊,彭军..丹参乙酸镁抗大鼠心肌缺血再灌注细胞程序性坏死的作用及机制[J].中国动脉硬化杂志,2017,25(6):571-575,5.基金项目
国家自然科学基金项目(81373409、81573430) （81373409、81573430）
湖南省教育厅科研基金项目(17C0204) （17C0204）
