中国动脉硬化杂志2017，Vol.25Issue(6)：576-581,6.
胆固醇对血管平滑肌细胞增殖及迁移的作用
Effects of cholesterol on proliferation and migration of vascular smooth muscle cells
摘要
Abstract
Aim To study the effects of cholesterol (CH) on proliferation and migration of vascular smooth muscle cells (VSMC).Methods VSCMs were treated with 12.5 mg/L,25.0 mg/L,50.0 mg/L CH.VSMC activity were analyzed by MTT kit,the percentage of DNA synthesis were detected by EdU kit,the percentage of S phase cell numbers were measured by flow cytometry and VSMC migrations were detected by scratch assay.The expression of miR145 was analyzed by real-time quantitative PCR.Results 12.5 mg/L,25.0 mg/L,50.0 mg/L CH can increase VSMC proliferation,migration and the percentage of S phase cell numbers (P＜0.05).25.0 mg/L,50.0 mg/L CH promoted DNA synthesis percentage of VSMC,decreased the expression of miR-145 in VSMC (P＜0.05).Conclusion Cholesterol increased the percentage of S phase VSMC number,cells proliferation,DNA synthesis and migration,and miR-145 may be involved in these processes.关键词
胆固醇/血管平滑肌细胞/增殖/miR-145Key words
Cholesterol/Vascular smooth muscle cells/Proliferation/miR-145分类
医药卫生引用本文复制引用
束波,刘志江,钱民章..胆固醇对血管平滑肌细胞增殖及迁移的作用[J].中国动脉硬化杂志,2017,25(6):576-581,6.基金项目
贵州省中医药管理局中医药、民族医药科学技术研究课题(QZYY-2015-152) （QZYY-2015-152）
遵义医学院2012招标课题(F-613) （F-613）
