中国动脉硬化杂志2017，Vol.25Issue(6)：582-589,8.
靶向PPARγ的miRNA在冠脉支架内再狭窄中的表达及意义
Expression and significance of the miRNAs target to PPARγ in coronary artery in-stent restenosis
摘要
Abstract
Aim To explore the key members of the miRNAs target to peroxisome proliferator-activated receptor γ(PPARγ),and reveal the clinical significance of which are closely related to in-stent restenosis (ISR).Methods Cytoscape and its plug-in was used to build the miRNAs-PPAR regulatory network to screen out which are the key miRNAs target to PPARγ.The expression of miRNAs in ISR group were detected by Real-time PCR and the clinical recognition ability of miRNAs on ISR patients were evaluated by ROC curve.Results By bioinformatics analysis,miR-27a/b has a strong regulatory effect on PPARγ,while miR-130a/b is able to regulate more genes in the network.Real-time PCR found that miR-27a/b and miR-130a were differentially expressed in the ISR group and no-ISR group (P＜0.05).ROC curve analysis showed that miR-27a has a certain clinical recognition ability to ISR (AUC =0.884,95％ CI 0.768～1.00,P＜0.001).Conclusion MiR-27a/b and miR-130a/b are key members of the miRNAs target to PPARγ,miR-27a/b and miR-130a were differentially expressed in ISR,miR-27a might have certain clinical diagnostic value in patients with ISR.关键词
冠心病/支架内再狭窄/miRNA/过氧化物酶体增殖物激活受体γ/生物信息学Key words
Coronary artery disease/In-stent restenosis/miRNA/PPARγ/Bioinformatics分类
医药卫生引用本文复制引用
张韶辉,赵永亮,魏广和,刘立新,杨国良,高振才,蔺跃栋,郭莹..靶向PPARγ的miRNA在冠脉支架内再狭窄中的表达及意义[J].中国动脉硬化杂志,2017,25(6):582-589,8.基金项目
山东省医药卫生科技发展项目(2014WS0515) （2014WS0515）
济宁医学院重点项目(JY2013KJ005) （JY2013KJ005）
