中国临床医学2017，Vol.24Issue(3)：328-333,6.DOI:10.12025/j.issn.1008-6358.2017.20170059
稳定表达绿色荧光蛋白肝癌大鼠肿瘤微转移时间窗的筛选
Screen of time window of stable expression of enhanced green fluorescent for transplanted hepatocellular carcinoma in Buffalo rats
摘要
Abstract
Objective:To discuss the time window of stable expression of enhanced green fluorescent for transplanted hepatocellular carcinoma in Buffalo rats.Methods:McA-RH7777/EGFP cells were subcutaneously implanted into the Buffalo rats' right thighs.Pieces of tumor tissue were implanted into the left lobes of the livers in 35 rats.The weights and serum albumin levels of rats were measured on day 7, 12, 18, 21, 26 after tumor implanted.The subsequent growth, metastasis of the implanted tumors were observed and evaluated with whole-body fluorescence optical imaging system, fluorescent microscopy.Results:The tumors were detected with MRI in all animals on day 7 after tumor implanted.There was no significant growth of weight within 7days, but increased significantly after and reached the peak value at day 21, then decreased.The mean tumor volumes was (13.36±2.90) mm3 on day 7, (162.5±69.71) mm3 on day 12, increased to (1 683.36±375.02) mm3 on day 26 after tumor implanted.With the fluorescent microscopy, micrometastasis tumors can be detected in lungs on day 21 after tumor implanted.Multiple small lung metastases were detected on day 26.The albumin levels on day 26 after tumor implanted were significantly lower than before (P<0.05).Conclusions:The period from 12 to 18 days in the models may be classified as the micrometastasis stage, and it becomes advanced liver cancer after 21 days.关键词
肝癌/分期/微转移/大鼠/绿色荧光蛋白Key words
hepatocellular carcinoma/staging/micrometastasis/rat/enhanced green fluorescent protein (EGFP)分类
医药卫生引用本文复制引用
张巍,钱晟,朱梁,周波,杨国威,刘嵘,瞿旭东,王建华,颜志平..稳定表达绿色荧光蛋白肝癌大鼠肿瘤微转移时间窗的筛选[J].中国临床医学,2017,24(3):328-333,6.基金项目
国家自然科学基金(81171432),复旦大学附属中山医院人才培养计划资助项目(2015ZSYYYXQN06,2015ZSYXGG18).Supported by National Natural Science Foundation of China (81171432) and Personnel Training Program of Zhongshan Hospital Affiliated to Fudan University (2015ZSYYYXQN06,2015ZSYXGG18). （81171432）
