中国临床药学杂志2017，Vol.26Issue(3)：194-197,4.
HPLC法同时测定人血清中3种抗癫痫药物的浓度
Simultaneous determination of three antiepileptic drugs in human serum by HPLC
姚韵茜 1苗彩云 1毛瑛瑛1
作者信息
- 1. 宁波市妇女儿童医院药剂科,宁波315012
- 折叠
摘要
Abstract
AIM To establish a simple method for the simultaneous determination of phenobarbital,phenytoin and carbamazepine in human serum by reverse phase high performance liquid chromatography (RP-HPLC).METHODS Estazolam was used as internal standard.The samples were evaporated to dryness under N2 stream after extracted by acetic ether and chloroform,then they were dissolved by methanol and analysized by HPLC with the Agilent Eclipse XDB-C18 column (150 mm ×4.6 mm,5 μm).The mobile phase was methanol-water(51:49),the detection wavelenth was 238 nm and the velocity was 1.0 mL · min-1.RESULTS The standard curve of phenobarbital,phenytoin and carbamazepine was linear in the concentration range of 2-80 mg · L-1,1.006-40.24 mg ·L-1 and 0.5-20 mg · L-1 respectively.The relative recovery rates were 101.10％,102.16％ and 100.84％,respectively.The extraction recovery rates were more than 95％.The RSDs of intra-day and inter-day were less than 6％.CONCLUSION The HPLC method is simple,rapid and accurate,and it is suitable for rapid drug monitoring of phenobarbital,phenytoin and carbamazepine.关键词
高效液相色谱法/苯巴比妥/苯妥英钠/卡马西平/血药浓度Key words
HPLC/phenobarbital/phenytoin/carbamazepine/serum drug concentration引用本文复制引用
姚韵茜,苗彩云,毛瑛瑛..HPLC法同时测定人血清中3种抗癫痫药物的浓度[J].中国临床药学杂志,2017,26(3):194-197,4.
