中国全科医学2017，Vol.20Issue(21)：2640-2643,2648,5.DOI:10.3969/j.issn.1007-9572.2017.21.016
白术七物颗粒剂对气阴两虚型慢传输型便秘模型大鼠结肠组织c-kit的影响及其作用机制
Effect and Mechanism of Atractylodes Qiwu Granules on C-kit Expression Level in Colon Tissues of STC Rats with Qi-yin Deficiency
摘要
Abstract
Objective To investigate the changes of c-kit expression level in colon tissues of STC model rats with qi-yin deficiency under the intervention of Atractylodes qiwu granules,and to explore the possible mechanism of action of Atractylodes qiwu granules in treating the disease.Methods This study was conducted from September to November 2015.A total of 70 SPF grade SD rats were randomized into the blank group(10 males and 10 females) and the model group(25 males and 25 females).Rats in the model group and blank group were subcutaneously injected 2.5 ml/kg of morphine hydrochloride injection,2.5 ml/kg of 0.9% sodium chloride injection,once daily for 45 consecutive days,respectively.The rats in the model group were gavaged with 13 g/kg of the solution with dissolved granules of pericarpium citri reticulatae viride,fructus aurantii and radix aconite carmichaeli on the 16th day of prescribing morphine hydrochloride injection,for 30 consecutive days to establish the STC rat models with qi-yin deficiency.The STC rat models with qi-yin deficiency were verified to be established successfully after the above 45d-intervention by examining the sacrificed 10 of the rats from the model group.The rest 40 rats in the model group were randomized into the model control group,high-dose Atractylodes qiwu granules group,low-dose Atractylodes qiwu granules group,and mosapride group,with 10 evenly composed of males and females in each group.After stopping the previous intervention for 7 days,the model control group,high-dose and low-dose Atractylodes qiwu granules groups,mosapride group and blank group were gavaged with solution of 0.9% sodium chloride injection,high-dose Atractylodes qiwu granules,low-dose Atractylodes qiwu granules,mosapride,0.9% sodium chloride,respectively.RT-PCR and immunohistochemistry were used to detect the expression levels of c-kit in the colon tissue samples taken from the rats of all the groups.Results The intestinal propulsion rate of model group was lower than that of blank group(P<0.05).The model control group had lower intestinal propulsion rate than low-dose and high-dose Atractylodes qiwu granules groups and mosapride group(P<0.05).The expression levels of c-kit mRNA in the model control group were lower than those in low-dose and high-dose Atractylodes qiwu granules groups and mosapride group(P<0.05).The expression levels of c-kit mRNA in low-dose Atractylodes qiwu granules group were lower than those in high-dose Atractylodes qiwu granules group(P<0.05).The model control group had lower expression levels of c-kit than high-dose Atractylodes qiwu granules group and mosapride group(P<0.05).Conclusion Atractylodes qiwu granules acts by increasing the c-kit level in colon tissues to treat STC with qi-yin deficiency.关键词
便秘/气阴两虚/大鼠/c-kitKey words
Constipation/Qi-yin deficiency/Rats/C-kit分类
医药卫生引用本文复制引用
林仁敬,鲁海燕,何永恒,聂晶..白术七物颗粒剂对气阴两虚型慢传输型便秘模型大鼠结肠组织c-kit的影响及其作用机制[J].中国全科医学,2017,20(21):2640-2643,2648,5.基金项目
国家自然科学基金资助项目(81302977) （81302977）
湖南省自然科学基金资助项目(13JJ3101) （13JJ3101）
