中国人兽共患病学报2017，Vol.33Issue(5)：418-422,5.DOI:10.3969/j.issn.1002-2694.2017.05.006
产丙酮酸棒状杆菌的鉴定及微生物学性状研究
Identification and microbiological characteristics of Corynebacterium pyruviciproducens
冯佳佳 1吴倩倩 1王保强 2孙铭艳 2陶元勇2
作者信息
- 1. 潍坊医学院医学检验学系纳米医学技术研究所,潍坊261053
- 2. 潍坊医学院附属医院检验科,潍坊261053
- 折叠
摘要
Abstract
The phenotype and genotype of the strains isolated from the sebaceous cysts were identified,and the biological characteristics and pathogenicity of the strains were described,which provided accurate etiological evidence for clinical diagnosis.Isolated strains were Gram stained and identified with VITEK 2 compact automatic microorganism analyzer.Antimiocrobial susceptibility testing was performed by K-B method.DNA was extracted from the isolated strains.The 16S rRNA fragment was amplified by universal primers PCR and sequenced.BLAST was used to compare the homology of the sequences to the 16S rRNA gene sequence in GenBank.Results showed that the isolated strain was identified as Kocuria roseus by VITEK 2 Compact,and then identified by 16S rRNA sequencing method as Corynebacteriium pyruviciproducens.Drug sensitive testing indicated that the isolates was sensitive to tetracycline,vancomycin,rifampin but resistant to penicillin,erythromycin,clindamycin,ciprofloxacin,levofloxacin,and compound new Ming,cefepime,imine pei south,gentamicin resistance.It is concluded that the phenotype of bacteria is not easy or inaccurate to identify;the 16S rRNA sequence is the most accurate determination method of identification.The Corynebacterium pyruviciproducens is a pathogenic bacterium causing the disease of the patient.At the same time,to improve the biochemical reaction information of Corynebacterium pyruviciproducens,the effective and feasible method of biological identification is also explored.关键词
产丙酮酸棒状杆菌/生物学特性/16SrRNAKey words
Corynebacterium pyruviciproducens/biological characteristics/16S rRNA分类
医药卫生引用本文复制引用
冯佳佳,吴倩倩,王保强,孙铭艳,陶元勇..产丙酮酸棒状杆菌的鉴定及微生物学性状研究[J].中国人兽共患病学报,2017,33(5):418-422,5.
