中国人兽共患病学报2017，Vol.33Issue(5)：423-426,4.DOI:10.3969/j.issn.1002-2694.2017.05.007
耐万古霉素肠球菌临床菌株毒力基因携带情况及差异
Distribution characteristics and diversity of virulence genes in clinical isolates of vancomycin-resistant enterococci
摘要
Abstract
The aim of this study is to determine the diversity of virulence genes carried by different vancomycin-resistant enterococci (VRE),which will provides a basis for studying pathogenic mechanism of VRE.Microdilution-based drug sensitivity test was applied to detect the vancomycin resistance of 490 Enterococcus faecium isolates and 862 Enterococcus faecalis isolates in Zhejiang area.The seven virulence genes (ace,asa1,cylA,efaA,esp,gelE and hyl) in the isolates of VRE were detected by PCR.According to the results of drug sensitivity test,10％ of the E.faecium isolates (49/490) and 0.8％ of the E.faecalis (7/862) were identified as VRE.In the vancomycin-resistant E.faecium isolates,five isolates were negative for any of the target genes and the other 44 isolates were positive for asa1,esp,gelE and hyl genes alone,in which the esp (73.5％,36/49) and hyl (53.1％,26/49) were the predominant genes and single or double virulence genes acted as the major carrying models.Except for the hyl gene,the vancomycin-resistant E.faecalis isolates were positive for the other six pathogenic genes,and the isolates could carry 3-6 pathogenic genes.All the data indicate that E.faeciurn is the major species of VRE in the local area,and the carrying rate,types and models of virulence genes in the vancomycin-resistant E.faecium and E.faecalis isolates are obviously different.关键词
耐万古霉素肠球菌/屎肠球菌/粪肠球菌/毒力基因Key words
vancomycin-resistant enterococci/Enterococcus faecium/Enterococcus faecalis/virulence genes分类
医药卫生引用本文复制引用
陈敏强,汪强,周永列,吕火烊,胡庆丰,葛玉梅..耐万古霉素肠球菌临床菌株毒力基因携带情况及差异[J].中国人兽共患病学报,2017,33(5):423-426,4.基金项目
浙江省医药卫生科技计划项目(2017KY004)资助 （2017KY004）
Supported by the Medical Scientific Research Foundation of Zhejiang Province (No.2017KY004) （No.2017KY004）
