中国人兽共患病学报2017，Vol.33Issue(5)：427-431,5.DOI:10.3969/j.issn.1002-2694.2017.05.008
miR-16在金黄色葡萄球菌脓毒症中的表达及意义探讨
Expression level of miR-16 in Staphylococcus aureus sepsis and its clinical significance
摘要
Abstract
We investigated correlation between the level of miR-16 expression and the severity of Staphylococcus aureus sepsis,and further explored its potentially clinical significance.Blood samples were collected from 32 patients,including each 8 cases of septic shock,severe sepsis and general sepsis,as well as 8 cases of healthy volunteers.Blood samples from 24 cases of healthy subjects with different ages were measured,and additionally 8 cases of blood samples from gram negative bacteria sepsis were also determined in current study as a control.Trizol solution for the whole blood lysis was added into blood samples,and followed by the extraction of microRNA.The expression levels of miR-16 in different groups were measured by fluorescence quantitative PCR,in which 2-Delta Ct method was used.SPSS software (version 13.0) was used to analyze the statistical differences between the groups,and further analyze the correlation between miR-16 value and the corresponding CRP and PCT values.Results showed that the expression level of miR-16 was negatively correlated with the severity of Staphylococcus aureus sepsis.There were statistically significant differences in experimental groups when compared with the control (P＜0.001),and there was also a statistically significant difference between each experimental group (P＜0.01).We found that the expression level of miR-16 was negatively correlated with CRP and PCT,the correlation coefficients were-0.561 and-0.769 respectively,and trend analysis showed that there was a significantly negative correlation.A significantly negative correlation was found between the miR-16 expression level and severity of sepsis,suggesting that miR-16 may serve as a biomarker for the severity of Staphylococcus aureus sepsis.关键词
miR-16/金黄色葡萄球菌/脓毒症Key words
miR-16/Staphylococcus aureus/sepsis分类
医药卫生引用本文复制引用
王家学,陈燕,刘小香,孙爱华,杨珺,吕火烊,周永烈..miR-16在金黄色葡萄球菌脓毒症中的表达及意义探讨[J].中国人兽共患病学报,2017,33(5):427-431,5.基金项目
浙江省自然科学基金(LY16H190006) （LY16H190006）
国家自然科学青年基金(31501581) （31501581）
浙江省教育厅一般科研项目(Y201534394) （Y201534394）
浙江医学高等专科学校科学研究计划项目(2015XZA02).Supported by Natural Science Foundation of Zhejiang Province (No.LY16H190006),the National Natural Science Foundation of China (No.31501581),the Scientific Research Foundation of Zhejiang Provincial Department of Education (No.Y201534394),and the Scientific Research Plan Project of Hangzhou Medical College (No.2015XZA02) （2015XZA02）
