中国生物防治学报2017，Vol.33Issue(3)：304-312,9.DOI:10.16409/j.cnki.2095-039x.2017.03.002
黑尾叶蝉NcPGRP基因克隆及表达模式分析
Molecular Cloning and Expression Pattern Analysis of Peptidoglycan Recognition Protein Gene in the Green Leafhopper, Nephotettix cincticeps (Hemiptera: Cicadellidae)
摘要
Abstract
To determine the function of peptidoglycan recognition protein in Nephotettix cincticeps (NcPGRP),we cloned the open reading frame cDNA nucleotide sequence of NcPGRP.The sequence is 552 bp in length,encoding a 19.9 kD protein but without signal peptide.NcPGRP has three Zn2+-binding and amidase catalyzing sites and one DAP-type peptidoglycan recognition site.Polygenetic tree analysis did not cluster NcPGRP and PGRPs of other species into one group.The real-time PCR results indicated that transcription level of NcPGRP was up regulated after injection ofEscherichia coli DH5α and Micrococcus luteus.Expression ofNcPGRP mRNA could be detected in all tissues including head,gut,fat body,Malpighian tubes,ovary,testis and cuticle,but was the highest in the head.The transcription level of NcPGRP was down regulated in RDV-infected planthopper.The results suggest that RDV-infected N.cincticeps exhibits a lower resistance to pathogenic bacteria infection,which may be conductive to the symbiosis between N.cincticeps and RDV.关键词
黑尾叶蝉/肽聚糖识别蛋白/水稻普通矮缩病毒/表达模式/免疫Key words
Nephotettix cincticeps/peptidoglycan recognition protein (PGRP)/Rice dwarf virus (RDV)/expression pattern/immunity分类
农业科技引用本文复制引用
黄天宇,方琦,王桂荣,林克剑,叶恭银..黑尾叶蝉NcPGRP基因克隆及表达模式分析[J].中国生物防治学报,2017,33(3):304-312,9.基金项目
国家“973计划”(2014CB138404) （2014CB138404）
