中国生物防治学报2017，Vol.33Issue(3)：364-370,7.DOI:10.16409/j.cnki.2095-039x.2017.03.010
球孢白僵菌有性世代培养及子代毒力稳定性鉴定
Development of Teleomorph from Beauveria bassiana and Virulence Stability of Its Progeny
摘要
Abstract
Strains selected by improved and typical mating practice are very important to enhance sexual reproduction ofBeauveria bassiana.Gene flow and evolution can occur during the stage of teleomorph.To study the inheritance of B.bassiana and solve the recession-related problem,we developed two novel methods for cultivating sexual generation,Cordyceps bassiana,using two kinds of media,pupae ofAntheraea pernyi and brown rice.The results showed that BbDPSD5 inbred strain of B.bassiana which possessed two mating type genes,MAT1-1-1 and MAT1-2-1,could develop into C.bassiana through these two media.No fruit bodies,however,were developed in other combinations,i.e.BbOFDH1-5×BbDPSD2,BbOFDH1-5×BbDPSD5,and BbDPSD2 ×BbDPSD5,on the two media.Mating type genes and partial segments of rDNA-ITS gene were amplified and sequenced from the cloning strains of ascospores.The results indicated that the rDNA-ITS gene and MAT1-2-1 gene from strains of ascospores had 100％ homologous with their parental type,and no MAT1-1-1 gene was detected.The results were consistent between the two media treatments.There was no significant difference on mortality between the ascospore strains and BbDPSD5 strain in toxicity tests.The results also indicated that there was no decrease in toxicity and no recession on parental strain.关键词
球孢白僵菌/有性态/交配型/有性世代Key words
Beauveria bassiana/Cordyceps bassiana/mating-type/teleomorph分类
农业科技引用本文复制引用
张云月,张军,李丽莉,杜茜,王振营,汪洋洲,李启云..球孢白僵菌有性世代培养及子代毒力稳定性鉴定[J].中国生物防治学报,2017,33(3):364-370,7.基金项目
吉林省自然科学基金(20150101097JC) （20150101097JC）
吉林省科技厅重大攻关专项(20140203006NY) （20140203006NY）
公益性行业(农业)科研专项(201303026) （农业）
