中国生物防治学报2017，Vol.33Issue(3)：371-377,7.DOI:10.16409/j.cnki.2095-039x.2017.03.011
植物内生特基拉芽胞杆菌的分离、鉴定及防治西瓜枯萎病效果
Isolation and Identification of Plant Endophyte Bacterial Bacillus tequilens and Its Control Effect against Watermelon Fusarium Wilt
摘要
Abstract
For the purposes of selecting new biocontrol agents,bacteria wm031 was isolated from the tomato plants using the dilution plate method and its antifungal activity was tested by the dual plate assay.The strain wm031 was idemified based on morphological characteristics,16S rDNA sequence analysis,physiological and biochemical characteristics,and its antagonistic against plant diseases was investigated by greenhouse experiments.The results indicated that the strain wm031 exhibited broad-spectrum antagonism against the tested fungi,including Fusarium oxyporum f.sp.niveum,Sclerotinia sclerotiorum in oilseed rape,Bipolaris sorokinianum,Gaeumannomyces graminis var.tritici Walker,Phytophthora capsici,Sclerotinia allii Saw.,Fusarium moniliforme Sheld,Colletotrichum gloeosporioides,and Fusarium oxysporum f.sp.lycopersici.The strain wm031 was identified as Bacillus tequilens,and it could stably colonize in watermelon and rice plants.Strain wm031 demonstrated good control effects on watermelon Fusarium wilt caused by Fusarium oxyporum f.sp.niveum,with the efficacy of 74.6％,higher than that of chlorothalonil by 14.1％.Thus,the strain wm031 is a potential biocontrol agent to be applied in the field.关键词
特基拉芽胞杆菌/分离/生防菌/西瓜枯萎病Key words
Bacillus tequilens/isolation/biocontrol agents/Fusarium wilt of watermelon分类
农业科技引用本文复制引用
张猛,王琼,冯发运,袁建军,余向阳..植物内生特基拉芽胞杆菌的分离、鉴定及防治西瓜枯萎病效果[J].中国生物防治学报,2017,33(3):371-377,7.基金项目
江苏省农业科技自主创新基金项目(CX-15-1035) （CX-15-1035）
国家自然科学基金(31401779) （31401779）
江苏省自然科学基金(BK20140745) （BK20140745）
泉州市科技计划项目(2013Z39) （2013Z39）
