中国生物防治学报2017，Vol.33Issue(3)：378-384,7.DOI:10.16409/j.cnki.2095-039x.2017.03.012
甲基营养型芽胞杆菌AL7对棉花黄萎病的盆栽防治效果及定殖能力
Effect of Bacillus methylotrophicus AL7 against Cotton Verticillium Wilt and Its Colonization in Rhizosphere of Cotton
摘要
Abstract
The control effects of Bacillus methylotrophicus AL7 on cotton Verticillium wilt were investigated by pot experiments and its colonization ability in cotton root and rhizosphere soil was evaluated by transforming tetracycline resistance plasmid pGFP78 into AL7 and then observing by tetracycline resistance plate medium and confocal laser scanning microscope (CLSM).The results indicated that the control efficacy of strain AL7 on cotton verticillium wilt was 77.1％,with motility and the ability to produce biofilm.After transformed into strain AL7,tetracycline resistance plasmid pGFP78 has no significant negative influence on the growth of AL7.The colonization detection results showed that AL7 could colonize in cotton rhizosphere soil.Colonization peak reaches 6 × 106 cfu/g soil at five days after inoculation with AL7,and the colonization quantity reaches 2 × 106 cfu/g soil at eighty days after treatment.Strain AL7 also could colonize in cotton root organization.The colonization quantity reaches 6 × 104 cfu/g root.CLSM observation showed that the strain AL7 could also colonize into cotton root tissue with a large number at seven days after inoculation.关键词
甲基营养型芽胞杆菌/棉花黄萎病/定殖Key words
Bacillus methylotrophicus/cotton Verticillium wilt/colonization分类
农业科技引用本文复制引用
刘海洋,姚举,张仁福,王伟,余璇,王琦..甲基营养型芽胞杆菌AL7对棉花黄萎病的盆栽防治效果及定殖能力[J].中国生物防治学报,2017,33(3):378-384,7.基金项目
新疆自治区重点研发计划项目(2016B01001-6) （2016B01001-6）
国家棉花产业技术体系岗位科学家项目(CARS-18) （CARS-18）
公益性行业(农业)科研专项项目(201503112-6) （农业）
