中国生物防治学报2017，Vol.33Issue(3)：385-393,9.DOI:10.16409/j.cnki.2095-039x.2017.03.013
青枯病植物疫苗对番茄根系土壤微生物群落结构的影响
Effects of Avirulent Ralstonia solanacearum Strain as Plant Vaccine on Microbial Community in Rhizosphere Soil of Tomato
摘要
Abstract
In order to explore the effect of avirulent Ralstonia solanacearum strain FJAT-1458 as plant vaccine on microbial community structures in tomato rhizosphere soil,tomato plants were inoculated by FJAT-1458 with water as a control.Then the rhizosphere soil was sampled at 3,6,9,12,15,18 and 21 d after treatment,respectively.Phospholipid fatty acids (PLFAs) of soil samples were detected using Agilent 6890 N.The results showed that PLFAs composition and content in tomato rhizosphere soil were significantly altered by inoculation of FJAT-1458,which could be divided into decreasing type,unchanging type and increasing type.Further statistical analysis showed that the inoculation of FJAT-1458 could increase the content of total microbes,bacteria and fungi by 5.98％-47.36％,2.07％-58.07％ and 5.65％-74.42％,respectively.Growth of actinomycetes was inhibited at the beginning and then improved by FJAT-1458 inoculation.Moreover,inoculation of FJAT-1458 could increase the microbial community diversity in tomato rhizosphere soil,and the enhancement of Shannon-wiene index was the most significant,with the highest value of 13.32％ at 21 d after inoculation.Cluster analysis revealed that the microbe in tomato rhizosphere soil both in FJAT-1458 treatment and control could be divided into three communities at 3.28 and 4.62 Lance distance,but constitutes and characters of PLFAs between the two treatments were different.Principal component analysis demonstrated that the two treatments could also be distinguished into two different groups.All these suggested that inoculation of FJAT-1458 could alter microbial community structure in tomato rhizosphere soil and increase its diversity,and further improve the stability of soil micro-ecosystem and the ability for bacterial wilt disease control.关键词
植物疫苗/番茄/土壤微生物群落结构/磷脂脂肪酸Key words
plant vaccine/tomato/soil microbial community structure/PLFAs分类
农业科技引用本文复制引用
郑雪芳,刘波,朱育菁..青枯病植物疫苗对番茄根系土壤微生物群落结构的影响[J].中国生物防治学报,2017,33(3):385-393,9.基金项目
国家公益性行业(农业)科研专项(201303015) （农业）
福建省自然科学基金(2015J01103) （2015J01103）
福建省农业科学院青年人才创新基金(YC2016-15) （YC2016-15）
