中国生物防治学报2017，Vol.33Issue(3)：421-426,6.DOI:10.16409/j.cnki.2095-039x.2017.03.018
枯草芽胞杆菌YN145分离鉴定及抑菌活性
Isolation and Identification of Bacillus subtilis YN145 against Magnaporthe oryzae and Its Antimicrobial Activities
摘要
Abstract
In order to explore the microbial resources for controlling rice blast,caused by Magnaporthe oryzae,527 strains were isolated from healthy rice plants of the susceptible cultivar Xiangzaoxian 24 by the method of plate dilution.60 strains with antagonistic activity against the blast fungus were obtained.The strain YN145 showed the strongest inhibition activity on mycelium growth with the rate of 84.31％.Based on the morphological analysis,physiological and biochemical characteristics,and 16S rDNA phylogeny,the strain YN145 was identified as Bacillus subtilis.Biological activity test showed that YN145 still had the inhibition rate of more than 80％ on mycelium growth after 25 generations of subcultures.Fermentation supematant of the strain could inhibit the mycelium growth by the rate of 91.50％,92.35％,91.78％,90.93％ and 86.97％,respectively,after treated at 40,60,80,100 and 120 ℃ for 30 min.Meanwhile,the fermentation filtrate could inhibit the mycelium growth by the rate of 91.50％,92.92％,92.92％,92.35％ and 92.63％,respectively,after treated by pH 3,5,7,9 or 11 for 30 min.The results suggested that antifungal substance of YN145 may be resistant to high temperatures and anti-acid and alkali.Thus,the strain YN145 is worthy for further research and development as rice blast bio-control agents.关键词
稻瘟病菌/枯草芽胞杆菌/抑菌活性Key words
Magnaporthe oryzae/Bacillus subtilis/antimicrobial activities分类
农业科技引用本文复制引用
邹秋霞,任佐华,高诗涵,周瑚,赵建辉,刘二明..枯草芽胞杆菌YN145分离鉴定及抑菌活性[J].中国生物防治学报,2017,33(3):421-426,6.基金项目
公益性行业(农业)科研专项(201203014) （农业）
湖南省“十二五”重点学科(0904) （0904）
国家重点研发计划(2016YFD0300700) （2016YFD0300700）
